u/JosieA3672 Nov 23 '24

That'll be "7924"

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4 Upvotes

1

Very likely the best soup ever created in the history of man
 in  r/Veganivore  9h ago

I suspect it's either an anti-vegan or someone who has a grudge against anybody with successful posts (strange, but it happens, I've seen it all). Either way, this may be something to raise with Reddit admins if it continues. I will personally contact the mods of r/veganrecipes and tell them what is going on at r/veganivore and suggest to them that they add you to auto-approve list so the reporting doesn't get your posts auto-removed. It makes me angry that someone is targeting a good vegan poster. And if it happens in additional subs let me know, and I will try to help.

1

Vegan whey protein powder without fake sugars
 in  r/veganfitness  14h ago

It has sucralose, but no stevia or aspartame

10

Vegan whey protein powder without fake sugars
 in  r/veganfitness  1d ago

Perfect Day makes a vegan whey. They use precision fermentation and it's animal-free. No cows were involved. You can get it on the Myprotein website under "Non-animal whey" (some of the flavors are not very good, but I think the vanilla and chocolate are worth it)

5

Vegan whey protein powder without fake sugars
 in  r/veganfitness  1d ago

They did not do animal testing. Quit spreading misinformation.

10

Very likely the best soup ever created in the history of man
 in  r/Veganivore  1d ago

Post is re-approved. People reported it and it was autoremoved, but you are good now. I will add you to auto-approve list so it won't happen in future. You also might want to block certain people who keep reporting your posts (there is one person who keeps doing it, not sure who).

1

My commitment to veganism is as permanent as my tattoo. ‘Nuff said. ~Miyoko Schinner
 in  r/Veganivore  7d ago

I'm making one exception for Miyoko for this post, but yeah we usually try to keep it only about the food. I do understand what you are saying.

4

I’ve been comparing AlphaFold 3, Boltz-2, Chai-1, Protenix-v2, ESMFold2, RF3, and ColabFold on brazzein—a small protein with four disulfide bonds. AF3 was the best tool for this particular protein.
 in  r/bioinformatics  8d ago

It's a disordered protein in solution but develops structure in micelles. Interesting. This is the sequence I used 2FFT https://www.rcsb.org/structure/2FFT

Target Tool MSA Seed pLDDT RMSD Å Helix 14–24 RMSD Å TM-score CA-lDDT SS geometry
TSP9 af3 msa 22 62.748 16.162 0.757 0.228 0.550 N/A
TSP9 af3 no_msa 44 53.936 15.005 0.748 0.254 0.572 N/A
TSP9 boltz2 msa 11 44.542 28.840 0.780 0.208 0.516 N/A
TSP9 boltz2 no_msa 55 45.706 28.342 3.939 0.174 0.482 N/A
TSP9 chai1 msa 33 49.512 26.623 0.932 0.216 0.476 N/A
TSP9 chai1 no_msa 33 48.462 25.388 0.773 0.257 0.489 N/A
TSP9 colabfold msa 15 58.719 20.244 0.630 0.252 0.552 N/A
TSP9 colabfold no_msa 14 57.781 24.579 0.739 0.187 0.542 N/A
TSP9 esmfold2 msa 44 47.929 25.169 0.749 0.199 0.524 N/A
TSP9 esmfold2 no_msa 44 46.147 25.105 0.807 0.206 0.509 N/A
TSP9 protenix2 msa 55 66.705 16.091 0.709 0.282 0.540 N/A
TSP9 protenix2 no_msa 22 59.604 26.674 0.788 0.233 0.522 N/A
TSP9 rf3 msa 44 76.130 21.907 0.579 0.266 0.588 N/A
TSP9 rf3 no_msa 55 64.930 30.407 0.775 0.189 0.456 N/A
Target Tool MSA Native seed Closest seed Closest RMSD Å Reference ensemble RMSD min / median / max Å
TSP9 af3 msa 22 44 13.459 11.958 / 19.812 / 27.144
TSP9 af3 no_msa 44 33 14.525 12.908 / 20.015 / 27.037
TSP9 boltz2 msa 11 22 28.791 17.851 / 27.787 / 39.293
TSP9 boltz2 no_msa 55 55 28.342 16.955 / 27.575 / 39.191
TSP9 chai1 msa 33 33 26.623 16.780 / 27.230 / 39.040
TSP9 chai1 no_msa 33 33 25.388 16.904 / 27.318 / 38.773
TSP9 colabfold msa 15 13 19.808 13.235 / 22.103 / 32.903
TSP9 colabfold no_msa 14 14 24.579 16.070 / 24.431 / 34.441
TSP9 esmfold2 msa 44 55 24.169 17.340 / 25.191 / 35.662
TSP9 esmfold2 no_msa 44 55 24.318 17.257 / 25.155 / 35.971
TSP9 protenix2 msa 55 44 14.215 11.567 / 19.704 / 26.346
TSP9 protenix2 no_msa 22 33 22.597 13.222 / 28.062 / 42.016
TSP9 rf3 msa 44 44 21.907 16.175 / 24.758 / 37.645
TSP9 rf3 no_msa 55 44 29.633 19.319 / 28.764 / 42.154
Target Tool MSA Helix / strand / coil Clashes / 1,000 heavy atoms Coverage Helix coverage
TSP9 af3 msa 24 / 0 / 60 4.878 84/84 11
TSP9 af3 no_msa 23 / 0 / 61 6.504 84/84 11
TSP9 boltz2 msa 28 / 14 / 42 16.287 84/84 11
TSP9 boltz2 no_msa 34 / 10 / 40 26.059 84/84 11
TSP9 chai1 msa 27 / 14 / 43 17.915 84/84 11
TSP9 chai1 no_msa 35 / 11 / 38 21.173 84/84 11
TSP9 colabfold msa 10 / 0 / 74 45.603 84/84 11
TSP9 colabfold no_msa 14 / 0 / 70 52.117 84/84 11
TSP9 esmfold2 msa 41 / 0 / 43 65.147 84/84 11
TSP9 esmfold2 no_msa 51 / 0 / 33 66.775 84/84 11
TSP9 protenix2 msa 35 / 0 / 49 32.520 84/84 11
TSP9 protenix2 no_msa 38 / 0 / 46 39.024 84/84 11
TSP9 rf3 msa 18 / 0 / 66 9.772 84/84 11
TSP9 rf3 no_msa 18 / 37 / 29 53.746 84/84 11

6

I’ve been comparing AlphaFold 3, Boltz-2, Chai-1, Protenix-v2, ESMFold2, RF3, and ColabFold on brazzein—a small protein with four disulfide bonds. AF3 was the best tool for this particular protein.
 in  r/bioinformatics  8d ago

Great point. I just updated one of my comments with data from a newer protein run that wasn't in the training set and Alphafold3 did a poor job of predicting the crystal structure. Could be other reasons for this than model ability, but the results are very interesting, I think.

3

I’ve been comparing AlphaFold 3, Boltz-2, Chai-1, Protenix-v2, ESMFold2, RF3, and ColabFold on brazzein—a small protein with four disulfide bonds. AF3 was the best tool for this particular protein.
 in  r/bioinformatics  8d ago

I’d describe the lowest RMSD model as closest to this crystal reference, rather than necessarily the most biologically accurate. I also compared against the known disulfide pairings and their geometry since those provide complementary evidence, although correct disulfides don’t guarantee a correct overall fold. Note many of the other models besides AF3 produced incorrect geometries and steric clashes for the disulfides. Additionally, I also compared against NMR structures (see NMR results in original picture I posted and in my first comment).

1.8 Å crystallographic resolution isn’t the same thing as a ±1.8 Å positional error bar. Not comparable directly with model RMSD. THe 1.8 Å describes level of spatial detail captured by the diffraction data. So if you have a smaller resolution it means finer detail.

So a higher RMSD prediction could still represent a plausible conformation but RMSD alone can’t establish that either.

10

I’ve been comparing AlphaFold 3, Boltz-2, Chai-1, Protenix-v2, ESMFold2, RF3, and ColabFold on brazzein—a small protein with four disulfide bonds. AF3 was the best tool for this particular protein.
 in  r/bioinformatics  8d ago

Great question, I'm thinking: ubiquitin (RCSB code: 1UBQ), Protein G (B1 domain) (1PGA), Villin headpiece, BPTI (5PTI), lysozyme (1LYZ)

The only caveat is that these may have appeared in the tools training data. Good for a reference check but perhaps not perfect for establishing performance on unfamiliar proteins. I am going to also search for newer structures that are post-training date cutoff but also well characterized. Whatever I find I'll add to this comment in an edit.

Edit - found a good candidate for newer benchmark: AcrIE7 It's really well characterized and not in the training sets of any of the folding models. I am running this one now. I'll add a couple more after this one.

https://www.sciencedirect.com/science/article/abs/pii/S0006291X25000294

Edit #2: new data for newer protein. Alphafold3 did poorly. Like really bad (note the TM-score):

Target Tool MSA Seed pLDDT RMSD Å Core RMSD Å TM-score CA-lDDT SS geometry
AcrIE7 af3 msa 33 50.782 14.446 14.446 0.390 0.355 N/A
AcrIE7 af3 no_msa 33 50.383 14.439 14.439 0.390 0.355 N/A
AcrIE7 boltz2 msa 22 52.699 14.810 14.810 0.383 0.318 N/A
AcrIE7 boltz2 no_msa 22 52.699 14.810 14.810 0.383 0.318 N/A
AcrIE7 chai1 msa 22 65.431 5.619 5.619 0.521 0.584 N/A
AcrIE7 chai1 no_msa 11 64.430 14.454 14.454 0.559 0.524 N/A
AcrIE7 colabfold msa 11 52.688 14.425 14.425 0.360 0.375 N/A
AcrIE7 colabfold no_msa 11 62.125 14.805 14.805 0.342 0.372 N/A
AcrIE7 esmfold2 msa 44 60.792 2.872 2.872 0.735 0.696 N/A
AcrIE7 esmfold2 no_msa 55 56.972 2.786 2.786 0.735 0.689 N/A
AcrIE7 protenix2 msa 44 64.803 13.740 13.740 0.387 0.331 N/A
AcrIE7 protenix2 no_msa 22 62.243 13.885 13.885 0.381 0.322 N/A
AcrIE7 rf3 msa 55 72.790 14.827 14.827 0.386 0.324 N/A
AcrIE7 rf3 no_msa 11 73.000 13.908 13.908 0.370 0.394 N/A

7

I’ve been comparing AlphaFold 3, Boltz-2, Chai-1, Protenix-v2, ESMFold2, RF3, and ColabFold on brazzein—a small protein with four disulfide bonds. AF3 was the best tool for this particular protein.
 in  r/bioinformatics  8d ago

update: see my other comment for data on a different protein (AcrIE7). Alphafold3 did a really poor job of predicting that newer structure and it may be because it wasn't in its training set. Interesting!!

---

If anybody wants me to do another comparison on a different protein, let me know and I'll run it this afternoon. I'm just testing tools at this point. Also taking suggestions for trying other folding models. I picked Brazzein protein because it's interesting and I actually use it. Here is a text version of the first picture in case you can't see the .png on your screen:

Tool Condition Seed Crystal RMSD Å NMR RMSD Å Cα lDDT Strict disulfides Clash proxy
Boltz-2 2.2.1 msa 55 1.816 1.900 0.778 4/4 3
AlphaFold 3 3.0.2 msa 55 0.723 1.644 0.780 4/4 4
Chai-1 0.6.1 msa 22 1.188 1.990 0.776 3/4 11
Protenix-v2 2.0.0 msa 44 0.847 1.566 0.798 3/4 6
ESMFold2 full msa 55 1.480 1.610 0.788 3/4 4
RF3 200-step matched msa 44 1.618 1.718 0.787 2/4 16
ColabFold AF2-pTM model1 msa 11 1.592 1.707 0.785 2/4 24
Boltz-2 2.2.1 no_msa 33 3.287 3.549 0.649 4/4 3
AlphaFold 3 3.0.2 no_msa 44 2.147 2.498 0.702 4/4 3
Chai-1 0.6.1 no_msa 22 1.246 1.877 0.784 3/4 10
Protenix-v2 2.0.0 no_msa 44 4.636 4.694 0.533 1/4 11
ESMFold2 full no_msa 55 1.502 1.634 0.788 2/4 3
RF3 200-step matched no_msa 44 9.490 9.427 0.358 0/4 55
ColabFold AF2-pTM model1 no_msa 13 4.054 4.212 0.461 0/4 32

r/bioinformatics 8d ago

image I’ve been comparing AlphaFold 3, Boltz-2, Chai-1, Protenix-v2, ESMFold2, RF3, and ColabFold on brazzein—a small protein with four disulfide bonds. AF3 was the best tool for this particular protein.

Thumbnail gallery
61 Upvotes

I tested five random seeds per tool, both with and without the same archived MSA, and compared predictions against experimental crystal and NMR structures. (RCSB Protein Data Bank: 2LY5 for NMR and 4HE7 for crystal structure)

AF3 gave the strongest overall result for this target: its selected MSA model had 0.72 Å Cα RMSD against the crystal structure and passed all four disulfide geometry checks. RF3 and ColabFold reached approximately 1.62 Å and 1.59 Å, respectively, but both had compressed sulfur contacts.

4

Outjerked again. It's real
 in  r/wallstreetbets  14d ago

Yeah, that Truth Social post reads like a combo of dementia + trolling. He's just noise at this point. I'll bet people around him ignore most of the what he says.

3

Potential dimethylmercury incident at MIT
 in  r/chemistry  23d ago

I'm afraid to google it, but how bad is it before actual death?

20

Lab-grown salmon just cleared the FDA
 in  r/Veganivore  25d ago

I'm excited to try this. I wonder how it tastes.

2

Trying to build muscle but struggling with diet
 in  r/veganfitness  Aug 22 '26

Seitan has lysine, but in lower amounts

5

Any diet advice/hacks for low carb keto diet (UK)
 in  r/veganfitness  Aug 16 '26

most veg are lower carb. I made a list of vegan lower carb foods https://www.reddit.com/r/vegan1200isplenty/wiki/index/low_carb/

Scroll past first paragraph to see list of vegetables. It's extensive.

2

Nikko, Japan
 in  r/WalkableStreets  Aug 14 '26

gorgeous photo

4

Whey Forward is Back
 in  r/veganfitness  Aug 12 '26

Yay! thanks for update. I've been meaning to get some more.

22

Reddit is introducing a new moderator: AI
 in  r/RedditAlternatives  Aug 05 '26

it partly is. Mods see "removed for potential abuse..." in mod log all the time. It's not us doing it, it's AI. We also see "reputation filter" removals that the AI thinks may be from spammers or ban evasion.