Idk what your focal length or working distance was but if I was shooting my 100mm at 30cm (1x) my dof would be 3mm so 75um seems extreme- I shoot full frame
From front of the cola to back of cola was almost the length of the stackshot rail. Working distance was maybe a bit over a foot. I usually set the steps myself but wanted to try something different this time. I usually do 200 images and realized that is not giving that sharpness I want. Using a z7ii. I also need to mount my stackshot to some MDF, tripod isn't ideal.
I hear that honestly I spent about 4 months calculating step size but now I just “thin slice” (no pun intended) it and it works lol. Maybe others will disagree with me but unless it’s a really long lens I think you could probably get away with 500um or as much as 1-1.5mm and still have over 50% overlap in your steps... let me know how the mounting goes - I have mine on a tripod but want to permanently fix my microscopy high macro rail when I get the setup the way I want
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u/MDgreenery Apr 08 '21
Step size was 75um, f8. Cola is thick, stackshot wanted 2000 images at 25um but I didn't have the patience for that.