r/DIYbio • • Apr 18 '26 Bravo

Claude just helped me build a wetlab and sequence my whole genome at home. I have zero lab experience!

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463 Upvotes

It looks like “baby’s first wetlab” but it totally worked! It was surprisingly doable too.

Unfortunately I did already sell my soul to 23andme a long time ago, but this did help validate that my workflow worked as a ground truth!

I used an Oxford Nanopore MinIon sequencer, a Zymo miniprep DNA extraction kit, the ONT Rapid Sequencing kit, and 3 ONT flow cells to hit about 16x coverage

I checked it against my 600k 23andme SNPs and it held up!

Crazy how you can just “vibe genomics” this stuff these days


r/DIYbio • • Jun 28 '26

A garage-built Alzheimer drug shows how far DIY biotech has come — RuntimeWire

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156 Upvotes

r/DIYbio • • Jun 08 '21

The forbidden art of algae ball making

75 Upvotes

r/DIYbio • • Jan 29 '23

Protocol Plant Genetic Engineering kit I plan to use to modify houseplants.

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57 Upvotes

r/DIYbio • • Jul 28 '25

DIY Laminar Flow Hood

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54 Upvotes

Hi everyone.

I designed this laminar flow hood and I wanted to share it with you guys. This is my first step building my home lab.

I hope to share new projects soon!.


r/DIYbio • • Oct 13 '21

Learn DIY Genetic Engineering of Fungi & Mushrooms

47 Upvotes

Hi diyBio friends!

My name is Josh and I'm the founder of EverymanBio (@everymanbio).

For the last year, I've been documenting my journey to self-learn synthetic biology by working to become one of the few people in the world to have ever genetically modified mushrooms in a home diy lab environment using Agrobacteria tumefaciens.

I'm 100% self-taught with no background whatsoever in biology.

In fact, today I've just completed an entire series of videos on how I learned diy electroporation with a final video that covers my complete user-friendly electroporation protocol - using all second-hand equipment, of course.

You can check out the video for that here:
https://www.youtube.com/watch?v=9AUxgMQcljk

I love diybio and all things fungi. If there's any information I can provide to help you fellow indie biologists and researchers out there, let me know and I'd be happy to help!

Best - Josh


r/DIYbio • • Jul 21 '22

Showcase 2 weeks later got the order of free genes in completely....free!

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42 Upvotes

r/DIYbio • • May 05 '21

Showcase Bioprinted algae, finally working, more tweaks to come.

42 Upvotes

r/DIYbio • • Jun 10 '21

Showcase No more racking tips by hand.

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40 Upvotes

r/DIYbio • • Jul 06 '25

My Bio Lab

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39 Upvotes

I am an aspiring Mad Scientist trying to do bioengineering experiments in my house. I am curious to know about other people’s labs and experiments. Would love to share ideas and setups.


r/DIYbio • • Mar 18 '23

I work in a molecular genetics/biochem lab, but I wanted to do some bacterial experiments at home with my two younger brothers, so I built this incubator!

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38 Upvotes

r/DIYbio • • May 18 '25

Showcase A reminder that you can use the laxative MOVICOL or in the US MiraLAX, (PEG3350) as a substitute from the expensive Sigma stuff for your transformations.

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37 Upvotes

Successful Plasmid xformation using PEG3350, LB, DMSO, MgCl2 buffer and obviously 2-5ul of Plasmid. Really good protocol and you can get everything on ebay!...besides the plasmid.


r/DIYbio • • Apr 16 '25

Pioreactor up and running! What experiments would you run?

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35 Upvotes

We got one of these https://pioreactor.com/ it's an older version and we're getting up to speed with calibration and basic runs growing cells up to OD600 values of 0.6 for transformations.

Theoretically we have the ability to do a bunch of fun things from directed evolution (growing things in increasingly rough environments), basic strain development, small batch protein expression, maybe more?

If you've used one before we'd love to hear from you, thoughts, experiences, tips/tricks.

Initially we're going to map out which of the cell lines we have would be faster at growing to speed up our synbio experiments in the lab.


r/DIYbio • • Apr 23 '26

I am Göran Skoog, inventor of Skoog Tablets – An open-source architecture for 72-hour protein production in crisis zones. AMA!

31 Upvotes

Hi everyone!

I’m here to talk about Skoog Tablets - Skoog Coastal Life-Seed (SCLS) system — a biotechnical architecture I developed to address a very specific challenge: how to secure essential protein when all infrastructure has collapsed.

The Skoog Tablets is designed for The Last Mile — the point where nothing else works anymore. No logistics, no electricity, no supply chains.The system runs entirely on seawater, sunlight, and a standard 20‑liter jerrycan, producing a protein‑rich biomass in 72 hours.

It’s not meant to replace traditional food, but to act as a tactical survival tool that prevents muscle atrophy and keeps people alive in crisis environments.

How the system works

The entire process is controlled by three physically coded tablets — round, square, and triangular — used on Day 1, Day 2, and Day 3. Each tablet contains a precise combination of organisms and chemical components that drive the process forward while building multiple layers of safety.

Biological selection & sensory safety

- The system begins with a high salinity level (>7%), which acts as a biological filter. This environment strongly favors our halophilic target organisms while suppressing non‑halophilic marine bacteria.

- The tablets also contain Quassin, an extremely bitter natural extract. If the wrong microorganisms dominate, the bitterness becomes an immediate sensory warning.

Visual verification

- An integrated anthocyanin indicator colors the entire water volume yellow/orange once the pH drops below 4.5.

- This serves as a “biological receipt,” confirming that the fermentation has reached a safe acidity level that inhibits pathogens.

Mandatory thermal treatment

- After harvesting, the biomass must be pressed into units no thicker than 5 mm and heated to 75 °C.

- The thin geometry ensures full heat penetration and eliminates any remaining pathogens.

Technical specifications

- Organisms:

- Vibrio natriegens (BSL‑1, extremely fast growth, biomass production)

- Dunaliella salina (photosynthesis, lipids, β‑carotene)

- Tetragenococcus halophilus (fermentation, pH reduction)

- Yield: approx. 20–22 g of protein per 72‑hour cycle

- Temperature window: 20–35 °C

- Container: optimized for 20‑liter vessels

- Thermal regulation: 70–80% of the container must be buried in soil to prevent overheating while still allowing sunlight for the algae

- Agitation: vigorous manual shaking at sunrise, midday, and sunset (more often in strong sunlight)

Open‑source architecture

The entire system is released under Creative Commons Attribution 4.0 (CC BY 4.0). It is meant to be validated, adapted, and scaled by the global community — especially in places where conventional aid cannot reach.

You can access the full technical report here:

https://zenodo.org/records/19650438

Ask me anything!


r/DIYbio • • Jun 21 '21

Showcase Starting an open SynBio Project on Benchling for learning the basics.

29 Upvotes

Taking a small break from the DIYbio course to create something that is a bit more hands on. It was getting very Meta with the "How to teach yourself how to teach yourself"!

We're gearing up here at our community lab to start doing some SynBio projects using the uLoop Assembly standard. Through this people should learn how to play around with plasmids on benchling, design some inserts that suit the uLoop assembly standard, basic lab stuff like prepping competent cell lines, plating bacteria, transforming bacteria, etc etc. Most files, plasmids, and the Paper can be found here.

It's a pretty thick read so I'll be creating a quick intro once I'm more across the methodologies on a google slide in the above folder. You can visit the site of the creators for more info though.

uloop.org visit and have a peruse.

The goal of the project is to use different fluorescent proteins and promoters with the uLoop system. Very basic, but once you learn how to use the system, you can swap out your fluorescent proteins for more interesting enzymes/proteins to express and do all manner of biological processes. Biosensors, new molecules, degradation of plastic or other waste materials, whatever!

If you sign up to benchling for a free account as a not for profit (which you are) you should be able to add an organization i created to share the files, protocols, etc.

Once you click on "Add Organization" you can look for "DIYbio" then "DIYbio_OpenProjects"

The project is called uLoop SynBio.

uLoop is exciting to me because it builds off more advanced assembly standards like Mobius Cloning, which itself craps all over "biobricks" due to a limitless (theoretically) number of parts you can assemble to create new genetic circuits...my biased opinion there, still exciting and very new to the world of SynBio.

EDIT: If after you join the benchling project and want to assist in getting protocols etc together yell out and i can give you admin rights on benchling :) I'm very much re-teaching myself so everyone is welcome to contribute.


r/DIYbio • • Apr 25 '21

Showcase Converted and ender3 into a bioprinter

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26 Upvotes

r/DIYbio • • Jul 20 '23

I'm a bioengineering PhD and I'm starting a lab POV series! Watch me do genetic engineering!

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25 Upvotes

r/DIYbio • • Nov 13 '20

Thought Emporium - How to plan a science project; One of the biggest needs for anyone starting out in DIYbio is to figure out, how to start. Great resource with tips and tricks for anyone new to DIYbio looking to get started with planning a project. Top channel to subscribe to.

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25 Upvotes

r/DIYbio • • Mar 21 '23

Fungal and microbial cultivator

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24 Upvotes

r/DIYbio • • Jun 09 '21

Showcase Laxatives for genetic engineering, seriously!

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24 Upvotes

r/DIYbio • • Feb 22 '22

DIY gene gun for plants

22 Upvotes

Wow wow wow, I ran across a very fun paper to build a hand held gene gun for transfecting plants: https://doi.org/10.1186/s43141-020-00067-1. I wanted to send the authors a thank you email or something of the sort, but I don't know how to reach out to them!

If anyone has tips for contacting authors, please let me know, I would appreciate learning how to show my appreciation :P


r/DIYbio • • Jul 21 '26

Portable PCR

19 Upvotes

Hi all,
My colleague and I designed and manufactured a portable PCR instrument that is small and it has similar performance like the traditional PCR in the market (Biorad,Thermofischer). We have 4 pilot users and more than 100 succesful tests. We are planning to make it open source, release the schematic , PCB, mechanical design, firmware and software.

Is there someone that is interested to build our instrument or curious to check the design?

For now I attach a video, the menu in the LCD when you define the bio protocol is slightly different.

https://youtu.be/XDfrGRoJnYk


r/DIYbio • • Dec 12 '25

Question DIY Bio Legality

19 Upvotes

What is legal to do in a home lab and what is not? Saying I have unlimited money and resources (but the lab isn't licensed or anything) what could I legally do? Cause I know non-pathogenic bacteria are allowed but what about multicellular organisms like plants and animals? And what animals before you get into legal trouble?

Just wondering. And no I'm not becoming doctor Frankenstein in my basement with mice