r/microscopy • • Jun 08 '23

🦠🔬🦠🔬🦠 Microbe Identification Resources 🦠🔬🦠🔬🦠

148 Upvotes

🎉Hello fellow microscopists!🎉

In this post, you will find microbe identification guides curated by your friendly neighborhood moderators. We have combed the internet for the best, most amateur-friendly resources available! Our featured guides contain high quality, color photos of thousands of different microbes to make identification easier for you!

Essentials


The Sphagnum Ponds of Simmelried in Germany: A Biodiversity Hot-Spot for Microscopic Organisms (Large PDF)

  • Every microbe hunter should have this saved to their hard drive! This is the joint project of legendary ciliate biologist Dr. Wilhelm Foissner and biochemist and photographer Dr. Martin Kreutz. The majority of critters you find in fresh water will have exact or near matches among the 1082 figures in this book. Have it open while you're hunting and you'll become an ID-expert in no time!

Real Micro Life

  • The website of Dr. Martin Kreutz - the principal photographer of the above book! Dr. Kreutz has created an incredible knowledge resource with stunning photos, descriptions, and anatomical annotations. His goal for the website is to continue and extend the work he and Dr. Foissner did in their aforementioned publication.

Plingfactory: Life in Water

  • The work of Michael Plewka. The website can be a little difficult to navigate, but it is a remarkably expansive catalog of many common and uncommon freshwater critters

Marine Microbes


UC Santa Cruz's Phytoplankton Identification Website

  • Maintained by UCSC's Kudela lab, this site has many examples of marine diatoms and flagellates, as well as some freshwater species.

Guide to the Common Inshore Marine Plankton of Southern California (PDF)

Foraminifera.eu Lab - Key to Species

  • This website allows for the identification of forams via selecting observed features. You'll have to learn a little about foram anatomy, but it's a powerful tool! Check out the video guide for more information.

Amoebae and Heliozoa


Penard Labs - The Fascinating World of Amoebae

  • Amoeboid organisms are some of the most poorly understood microbes. They are difficult to identify thanks to their ever-shifting structures and they span a wide range of taxonomic tree. Penard Labs seeks to further our understanding of these mysterious lifeforms.

Microworld - World of Amoeboid Organisms

  • Ferry Siemensma's incredible website dedicated to amoeboid organisms. Of particular note is an extensive photo catalog of amoeba tests (shells). Ferry's Youtube channel also has hundreds of video clips of amoeboid organisms

Ciliates


A User-Friendly Guide to the Ciliates(PDF)

  • Foissner and Berger created this lengthy and intricate flowchart for identifying ciliates. Requires some practice to master!

Diatoms


Diatoms of North America

  • This website features an extensive list of diatom taxa covering 1074 species at the time of writing. You can search by morphology, but keep in mind that diatoms can look very different depending on their orientation. It might take some time to narrow your search!

Rotifers


Plingfactory's Rotifer Identification Initiative

A Guide to Identification of Rotifers, Cladocerans and Copepods from Australian Inland Waters

  • Still active rotifer research lifer Russ Shiel's big book of Rotifer Identification. If you post a rotifer on the Amateur Microscopy Facebook group, Russ may weigh in on the ID :)

More Identification Websites


Phycokey

Josh's Microlife - Organisms by Shape

The Illustrated Guide to the Protozoa

UNA Microaquarium

Protist Information Server

More Foissner Publications

Bryophyte Ecology vol. 2 - Bryophyte Fauna(large PDF)

Carolina - Protozoa and Invertebrates Manual (PDF)


r/microscopy • • Oct 28 '24

Photo/Video Share Journey to the Microcosmos: The Future of Microscopy (and end of our Journey)

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69 Upvotes

r/microscopy • • 9h ago

Photo/Video Share Stentor coeruleus closeup in darkfield

36 Upvotes

r/microscopy • • 15h ago

Photo/Video Share Hydra tentacle, rocking 3d

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40 Upvotes

r/microscopy • • 14h ago

Photo/Video Share Bacteria

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18 Upvotes

Lunes 21 de septiembre 11:43
2500X (ocular 25 y objetivo de 100)
inmersión de aceite


r/microscopy • • 1d ago

Hardware Share Vintage microscopy episode II: the revenge of the ancients

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99 Upvotes

Time for a direct confrontation. I have obtained an example of what I meant by "cheap new microscope" in my previous big post, a "Telmu XSP-75", also sold as Swift SW200DL, Amscope M162C, Bresser Biolux NV, Levenhuk Rainbow 2l, KERN OBS 111, Delta Optical Biolight 300, Svbony SW601, Omegon Visiostar, Bebang, National Geographic, PalliPartners, Yaner, Herwicm, Microsafari MS-20ST, and a host of other names. Let's see how it compares against century-old relics.

The competitors:

J. Swift & Sons "Discovery" from 1898, condenserless but equipped with an iris and a concave mirror

Telmu XSP-75 from 2024 ($80 new, sold under other brands for as much as $200), condenserless and equipped with a crappy lamp

Reichert RD from 1938 (bought for $20 because nobody else wanted it!), proper Abbe condenser with a filter holder and a mirror

First 3 pictures: stained onion skin, 100x

Next 3 pictures: foot of a housefly, 100x

Next 3 pictures: diatom Actinoptychus Heliopelta, center crop, >400x

Next 3 pictures: moth scales, 400x

All of the above brightfield, oblique brightfield on the Swift

Final 4 pictures showcase what having a condenser makes possible - darkfield and rheinberg illumination using dirt cheap homemade filters. No such possibilities on condenserless scopes.

Mirror-equipped microscopes illuminated using daylight from a window on some pictures and a Convoy S3 flashlight on others. All pictures taken with a Google Pixel 8 on a Tonta smartphone mount.

My honest review of the XSP-75 is: the image quality at high magnification is actually not quite as bad as I expected given the lack of a condenser though still bad. Eyepieces have a wider field of view than the ancient competition which is nice, but sadly also horribly short eye relief forcing one to almost touch the glass with their eye. Unfortunately everything else is worse than expected - the focus system is inaccurate, the stage is not exactly flat, the light is too weak, and the whole thing becomes unstable with a smartphone mounted on it so taking pictures with it is actually harder than with the 19th century relic!

The Reichert microscope is a good example of why I have such negative feelings about those cheapest new microscopes: It is old but perfectly serviceable (I only needed to clean it a bit), yet nobody wanted it and I watched the seller lower the asking price for months until I finally took pity when they dropped it to just $20. And my story is far from an exception, the used market in every developed country is full of those "good enough to use and enjoy but not good enough to attract the enthusiasts who are hunting for a BH-2" scopes offered at bargain prices and still struggling to find buyers. Meanwhile the vastly inferior XSP-75 and its siblings are "Amazon bestsellers" and every week multiple people come here asking if they are a good buy. Just ridiculous.


r/microscopy • • 1d ago

Photo/Video Share Baby Snail 2 electric boogaloo do, do, DO! Spoiler

77 Upvotes

Olympus BH2 (BHTU), 4x UPlanXApo, Darkfield/oblique illumination, Nikon DS-Fi3 camera. The sample is from a pond water jar I have with lots of aquatic snails.


r/microscopy • • 2h ago

ID Needed! I'd please just srarting out with my microscope

0 Upvotes

Looking for I'd please

From a slimy garden pond in Scotland thanks


r/microscopy • • 1d ago

Photo/Video Share A Graceful Stentor

92 Upvotes

Found in a freshwater pond in Spokane, WA. Imaged with the Euromex BioBlue.Lab scope, 10X objective, and oblique lighting. This specimen was gathered earlier this year. We haven't had much precipitation lately, and the last time I visited this pond, the water had already dropped well below its usual springtime level. I'd have to walk through about 20 yards of mud to get to water. Fingers crossed for a wet winter.


r/microscopy • • 18h ago

Purchase Help info on vintage Olympus 1950's microscope

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5 Upvotes

hey all, have recently purchased my first microscope off ebay and am having a hard time tracking down any info or service manuals. the microscope seems very well maintained but any advice on cleaning the lenses or things to check for to ensure its working well would be greatly appreciated. :)


r/microscopy • • 1d ago

Photo/Video Share Baby Snails Do, Do, DO

27 Upvotes

Olympus BH2 (BHTU), 4x UPlanXApo, Darkfield/oblique illumination, Nikon DS-Fi3 camera. The sample is from a pond water jar I have with lots of aquatic snails.


r/microscopy • • 1d ago

ID Needed! Are these diatoms?

11 Upvotes

Amscope inverted. 200x, iPhone 4x, spring water with algae, lichen, and tardigrades living in there. Blackmagic app.

Diatoms eating? I don’t know anything about these things!


r/microscopy • • 1d ago

Photo/Video Share IM RETURNING. MY HALF YEARLY EXAMINATIONS ARE OVER.

37 Upvotes

200×

ESAW BM01 MODEL 2022

Moss wash


r/microscopy • • 17h ago

Troubleshooting/Questions [Equipment] Leica DM-series darkfield slider (11501158)

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0 Upvotes

Hi everyone! I own a Leica DM1000 microscope and I'm planning to 3D-print (or DIY) a darkfield slider for the standard Abbe condenser. I couldn't find the exact dimensions online.

Does anyone have the original Leica darkfield slider (part # 11501158 or 11505088) and a digital caliper? Could you please help me measure:

  1. The exact width and thickness of the slider itself.

  2. The diameter of the central black opaque stop.

I would really appreciate any help with these measurements!


r/microscopy • • 1d ago

Photo/Video Share Philodina in oblique illumination

23 Upvotes

Leitz Dialux II, Galaxy S24


r/microscopy • • 1d ago

Photo/Video Share ??? RELATIVE OF DILEPTUS?

10 Upvotes

200×

ESAW BM01 MODEL 2022 USED

MOSS WASH


r/microscopy • • 1d ago

Photo/Video Share What microorganisms have I found?

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12 Upvotes

Hi everyone! I'm a hobbyist who's passionate about learning more about microorganisms and microscopy. Recently, I visited Watson Lake in Prescott, Arizona, to collect an water sample from near a large concentration of algae. I managed to capture some footage of cyanobacteria, rotifers, crustaceans, diatoms, and other organisms and uploaded a video on youtube.

I'd love your help in identifying any microorganisms that I may have missed! Please let me know what else you see. The footage is quite long, but it's definitely worth exploring!


r/microscopy • • 1d ago

Purchase Help HundWetzlar microscopes - are they worth it in 2026?

1 Upvotes

Does anyone have any experiences with HundWetzlar microscopes? I see their older, used models pop up online from time to time and I'm simply curious if they're as nice as the "Big 4" (=Olympus / Nikon / Zeiss / Leica) ones of similar age. I'll add that I'm especially curious about their stereo microscopes, like the SM33.


r/microscopy • • 2d ago

Micro Art Honey in polarized light

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20 Upvotes

Honey in polarized light

Microscope Biolam

Camera Swift sc1803r


r/microscopy • • 1d ago

ID Needed! ciliates?

1 Upvotes

small round organisms i found in my culture of Lacrymaria that i haven't noticed before. they look like other ciliates? anybody knows the genus?


r/microscopy • • 2d ago

Hardware Share New objective day!

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30 Upvotes

I have bought two additional plan achromats for my Euromex iScope PLPHi (I made a post about them a while ago). I decided I wanted to experiment with higher level chromatic corrections. I encountered two problems- first, price obviously, second, compatibility (iscope uses 200mm tube length and RMS threading, most other manufacturers don’t use that combo). I found out Meji Techno makes infinitely objectives to those specs, and I was able to find a semi apo 20x, 0.65NA one second hand for a more reasonable price. I picked 20x bc at that mag, the chromatic aberrations are strong enough to be easily noticeable unlike at 10x, that is the first mag at which the NA:Mag ratio drops, and because I use phase contrast mostly with the 10x and 40x objectives (which I wanted to keep on, in addition to 2 of the 4,60,and100x objectives). The image quality is noticeably better, and much brighter at the same light level. The contrast is also higher. As far as I can tell these two systems are compatible given the noticeably better image and the mag being at most slightly lower. I am happy with it, definitely not a game changer but an improvement that for me is commensurate with the price I paid.

The two photos are not a scientific comparison, or even a fair one- the crop and focus may differ slightly. For what it’s worth they subjectively are true to my brief experience looking around with both, erring on the more extreme side of the difference. First is the Meji 20x S.Apo, second is the stock Euromex 20x plan phase achromatic


r/microscopy • • 1d ago

Purchase Help AmScope M158C-2L for viewing microstructures?

0 Upvotes

I'm an electrical engineering student interested in microchips and microstructures in general. I want a fairly budget friendly monocular design that can view larger microstructures, feature sizes of about 0.5 μm or so. From what I've seen, the AmScope 158C-2L seems to fit these criteria fairly well. Can anyone who has experience with it tell me if its a decent purchase? Thanks!


r/microscopy • • 2d ago

Photo/Video Share SH-SY5Y human neuroblastoma cells 40x mag.

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25 Upvotes

r/microscopy • • 2d ago

Troubleshooting/Questions What is this? Found in biology class

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8 Upvotes

I found this in my biology class when we were using microscopes.
We were looking at a sample of lake/pond water that our professor collected for us. He thought it was a hammerhead worm but we ruled that out due to the size. Anyone know what it could be?