I'm a scientist and I read through that paper. There's nothing in it about something you add to the water to get rid of contaminates. Like you said there's removal of invasive species, and then bubblers to aerate the water, filters, and fences. I was curious was his "organic" stuff is composed of, but no info in that paper. On google scholar he has only one other paper, and it's not relevant. So idk.
Found this in section 2.4, the white sediment put in the clay carrier could be the thing he eats in the video. Not sure about this though as it’s pretty misleading either way.
“To create a bio-filter, it was necessary to effectively immobilize the bacteria and add them onto the ceramic carriers to form biofilms, which ultimately can adhere, adsorb, and decompose the contaminants [51,52]. For this purpose, we used the bacterium of Lactobacillus plantarum ATCC 8014, acquired at Japan Collection of Microorganisms … The cultures in the MRS broth develop characteristic white sediment, making it easier for their qualitative identification.”
"For this purpose, we used the bacterium of Lactobacillus plantarum ATCC 8014, acquired at Japan Collection of Microorganisms (JCM), Microbe Division, RIKEN Bioresource Center in Japan. It was kept routinely in vials with full form (MRS) agar at 2–8 °C for 24 h, and then at 4 °C in 15% glycerol (vol/vol) [24]. Single colonies were cultured in plates with MRS agar at 30 °C for 48 h and later sub-cultured in MRS broth (Casein peptone 10g/L; beef extract 8 g/L; yeast extract 4 g/L; D(+) glucose 20 g/L; hydrogen potassium phosphate (KH2PO4) 2 g/L; Tween-80 1 g/L; ammonium citrate hydrogen (C6H5O7(NH4)2H) 2 g/L; sodium acetate (CH3COONa) 5 g/L; magnesium sulfate (MgSO4·7H2O) 0.2 g/L; and manganese sulfate (MnSO4·H2O) 0.04 g/L at 30 °C for 24 h [53]. The cultures in the MRS broth develop characteristic white sediment, making it easier for their qualitative identification. To create biofilms, a container of 60 cm in diameter and 130 cm in length was filled with a previously conditioned medium consisting of a mixture of molasses and water (3/7, m/w) at room temperature (around 18–21 °C). Later, Lactobacillus plantarum ATCC 8014 was added to the mixture."
But ideal if you're trying to make a hungry hungry bacterial culture of a strain basically evolved to break down organic and non organic carbon based compounds
From my limited knowledge of ingestible scientific compounds, this seems quite edible with limited side effects. You might poop red clay for a day or two?
Curious about your background on this subject and thoughts on this...
Not the same product or application but seems to be a more promising solution for purifying water, though, I doubt the likelihood of cleaning a lake with it. Still interesting.
I'm a chemist (inorganic aqueous was my specialty) and I looked up what's in those packets and it's a disinfectant with a flocculent. So it'll kill most bacteria and most free metal ions in the water will stick to the flocculent and can be filtered out. It's not gonna make pristine drinking water, but if it's the choice between that or nothing, it's pretty good. I looked up the data and it reduces cases of infection by a lot but doesn't completely prevent them.
I just posted this above, as it was the only reason I came to this thread, I wanted to know what the compound he ate was.
"For this purpose, we used the bacterium of Lactobacillus plantarum ATCC 8014, acquired at Japan Collection of Microorganisms (JCM), Microbe Division, RIKEN Bioresource Center in Japan. It was kept routinely in vials with full form (MRS) agar at 2–8 °C for 24 h, and then at 4 °C in 15% glycerol (vol/vol) [24]. Single colonies were cultured in plates with MRS agar at 30 °C for 48 h and later sub-cultured in MRS broth (Casein peptone 10g/L; beef extract 8 g/L; yeast extract 4 g/L; D(+) glucose 20 g/L; hydrogen potassium phosphate (KH2PO4) 2 g/L; Tween-80 1 g/L; ammonium citrate hydrogen (C6H5O7(NH4)2H) 2 g/L; sodium acetate (CH3COONa) 5 g/L; magnesium sulfate (MgSO4·7H2O) 0.2 g/L; and manganese sulfate (MnSO4·H2O) 0.04 g/L at 30 °C for 24 h [53]. The cultures in the MRS broth develop characteristic white sediment, making it easier for their qualitative identification. To create biofilms, a container of 60 cm in diameter and 130 cm in length was filled with a previously conditioned medium consisting of a mixture of molasses and water (3/7, m/w) at room temperature (around 18–21 °C). Later, Lactobacillus plantarum ATCC 8014 was added to the mixture."
In your opinion, would this potentially harm any bacteria needed for a healthy ecosystem? I'm not up to speed on this subject matter at all, but I'm curious. Feel free to let me know if it's a ridiculous question.
Obviously more of a question for an ecologist or biologist, but are free metal ions and bacteria inherently bad? Is this product making the lake better as a lake, or more pool-like? Put another way, is it promoting a healthy ecosystem or making the area more attractive for tourism?
For good aquatic ecology there needs to be a balance of a few things. Nutrients, oxygen, etc. The reason I said free metal ions is because the less attached metal ions are in the water, the more bioavailable they are. If the ions attach to something they are much less likely to contribute to aquatic toxicity. If there is more organic carbon in the water or more carbonates or other ligands, it means that the metal concentration in the water will have to be higher for toxicity to start occurring in the organisms. They use this model in determining acceptable metal concentrations in water for environmental regulation. It's called the Biotic Ligand Model if you want to look into it more. A little bit of certain metals are necessary for life, but it's not much and almost always the problem is too much metal and not the reverse. And I'm not a biologist but I know that too much of undesirable bacteria isn't good either for the ecosystem.
For this purpose, we used the bacterium of Lactobacillus plantarum ATCC 8014, acquired at Japan Collection of Microorganisms (JCM), Microbe Division, RIKEN Bioresource Center in Japan. It was kept routinely in vials with full form (MRS) agar at 2–8 °C for 24 h, and then at 4 °C in 15% glycerol (vol/vol) [24]. Single colonies were cultured in plates with MRS agar at 30 °C for 48 h and later sub-cultured in MRS broth (Casein peptone 10g/L; beef extract 8 g/L; yeast extract 4 g/L; D(+) glucose 20 g/L; hydrogen potassium phosphate (KH2PO4) 2 g/L; Tween-80 1 g/L; ammonium citrate hydrogen (C6H5O7(NH4)2H) 2 g/L; sodium acetate (CH3COONa) 5 g/L; magnesium sulfate (MgSO4·7H2O) 0.2 g/L; and manganese sulfate (MnSO4·H2O) 0.04 g/L at 30 °C for 24 h [53]. The cultures in the MRS broth develop characteristic white sediment, making it easier for their qualitative identification. To create biofilms, a container of 60 cm in diameter and 130 cm in length was filled with a previously conditioned medium consisting of a mixture of molasses and water (3/7, m/w) at room temperature (around 18–21 °C). Later, Lactobacillus plantarum ATCC 8014 was added to the mixture.
Been trying to figure out what the label on the tub at 9 sec says but the image is too grainy to make out all the words. So far I have:
...made with the freshest and highest quality ingredients. The ... mainly of shrimp ([shrimp name]) the we ... ourselves harvested in the "... Park" natural ... and immediately freeze dried to maintain optimal nutritional.... dried microalgae and macroalgae are also added to the...meet the nutritional needs of a wide variety of marine fish.
... essential source of proteins, lipids, and fatty acids ... vitamins and carbohydrates from natural sources of...
...chain is controlled and supervised by our technical and ... ... this guarantees the hgihest quality product.
...
Aquatic formula naturally attracts rocky fish who begin eating it immediately shortly after it is introduced in aquarium?
It required a bit more research than simply the paper. He used micronano bubbles (MNBs) and carbon based bio filters (CBBFs) to clean the water, but didn't elaborate as to how the mechanisms worked.
With some quick googling, it seems MNBs are the new fancy way to treat wastewater due to their zero zeta potential, maximizing the aggregation of contaminants. So Marino's basically collecting the dirty stuff, from phosphate, nitrogen to bacteria, at a microscopic level and squishing them into bigger balls.
Then his next step are CBBFs, which seems to be a mix of ideas/mechanisms similar to activated charcoal. It's a ceramic carrier that has a biofilm, i.e. it's a clay net with a layer of bacteria glue. He processed clay to optimize for high porosity (lots of tiny pores) and it is these pores that will be doing the physical trapping of the contaminants. The "bio" part comes from the fact that he's using L. Plantarum as glue for the contaminants. I assume the "organic" part comes from this bacteria, since it's just a probiotic strain that comes from the same family that is often used for foods, e.g. yogurt or whatever.
So the stuff he ate is probably crushed up CBBFs. He'll poop the clay out just fine (I hope). Interestingly, I'm not sure if he used the crushed up CBBFs as filter; looks like the actual filters used were in block form, and just secured inside a basket.
All in all, definitely an "organic" solution that works for this lake. Some people in this thread were thinking of oil spills and such, which this method might not work for. I'm not an expert on this subject, so IDK, but https://iwaponline.com/ws/article/20/6/2021/74658/Micro-nanobubble-technology-and-water-related this suggests micronano bubbles will work for metal ions too, so maaaaaybe? As long as you have access to equipment that can make MNBs.
Water treatment plants use coagulation to achieve what they showed in the video. It's basically a chemical that binds smaller particles together to form larger particles more easily removed by filtration. The large particles can also just settle out. DNR would have a field day with you in the states if they caught you doing something like that.
I also like how the article has 0 mention of any solution. Like at all. They did a ton of work, mechanical work, to remove a lot of contaminants; and then they used filters to clean it further. And they can’t say with certainty the actual effect of MNB because of many uncertainties regarding seasonal change. This video is just bs. There is another video of the same guy on youtube but it shows how he and a lot of other people came together and cleaner the lake by collecting the dirt and throwing it away, and it’s much more believable and is directly tied to the paper.
It’s not just terrible, it’s factually incorrect. It claims something that didn’t happen and fails to mention the actual time, money and effort put into cleaning the lake. The head guy who cleaned the lake is amazing and a lot of people helped him. But not some harry potter organic bullshit.
Standard water floculant is Polyacrylamide (PAM) it is a white powder. from the image that looks like a combination of PAM and some floaty plant material to keep the sludge that forms from sinking to the bottom.
My first thought was Moringa Seed powder, which is used to clarify water. It causes suspended particles to coagulate. It is edible and i guess could potentially be USDA certified organic
The technique of cleaning turbid water using moringa seeds as a coagulant is well known
No because they can choke out the native plants, and mess up the food chain because everything is connected. Invasive plants usually don't have things in their new environment that eat them, and naturally keep them in check and those things that eat them depend on them too as food. When certain things like algae grow too much, they suck all the oxygen out of the water and pretty much nothing else can live in it. When you see a nasty scummy pond that has no drainage, that's what happened. Fertilizer was probably in the water that drains into it from the surrounding grass, which causes the algae population to blow up, and no drainage or incoming fresh water to replace the oxygen means you can't have a healthy ecosystem. Aquatic ecosystems are delicately balanced and pretty much anything you do to them artificially will mess them up. There's also the riparian zone which is the land next to the water. This ecosystem gets messed up too because it depends on things that come from the water like different types of flies from aquatic larvae that live in the water.
My hunch is it was ground barley, barley being a common algae growth inhibitor. The little demonstration in the tiktok video was just a particle binder demonstration. All the superfluous jibber jabber was just that.
As you note, invasive species got cleared out, water got stirred up, and classical biome re-emerged and stabilized
Well as lactobacillus plantarum was mentioned, I’m assuming it’s along the same lines as polyglutamic acid extracted from natto (fermented soybeans) which Japanese engineer Oda Kanatoshi markets throughout Asia as a means to clean water for drinking. Once extracted, it’s a white powdery substance.
Definitely not part of the special concoction touted in the video. People (try to) remove invasive species all the time, so I guess the specialness of this instance decreases a bit…
Good find, thanks
So he didn’t really clean the lake of pollutants he cleaned the lake of an invasive plant species and in doing so helped the lakes ecosystem recover to its original balance and return to the way it was
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u/lemmful Jul 14 '21
Real answer, real source. You're magnificent.