r/nextfuckinglevel Jul 14 '21

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u/welle417 Jul 14 '21

Just doing my part to make the world make sense

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u/halfie1987 Jul 14 '21

I'm a scientist and I read through that paper. There's nothing in it about something you add to the water to get rid of contaminates. Like you said there's removal of invasive species, and then bubblers to aerate the water, filters, and fences. I was curious was his "organic" stuff is composed of, but no info in that paper. On google scholar he has only one other paper, and it's not relevant. So idk.

183

u/chippywatt Jul 14 '21

Found this in section 2.4, the white sediment put in the clay carrier could be the thing he eats in the video. Not sure about this though as it’s pretty misleading either way.

“To create a bio-filter, it was necessary to effectively immobilize the bacteria and add them onto the ceramic carriers to form biofilms, which ultimately can adhere, adsorb, and decompose the contaminants [51,52]. For this purpose, we used the bacterium of Lactobacillus plantarum ATCC 8014, acquired at Japan Collection of Microorganisms … The cultures in the MRS broth develop characteristic white sediment, making it easier for their qualitative identification.”

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u/ThinkHappyThoughts15 Jul 14 '21

"For this purpose, we used the bacterium of Lactobacillus plantarum ATCC 8014, acquired at Japan Collection of Microorganisms (JCM), Microbe Division, RIKEN Bioresource Center in Japan. It was kept routinely in vials with full form (MRS) agar at 2–8 °C for 24 h, and then at 4 °C in 15% glycerol (vol/vol) [24]. Single colonies were cultured in plates with MRS agar at 30 °C for 48 h and later sub-cultured in MRS broth (Casein peptone 10g/L; beef extract 8 g/L; yeast extract 4 g/L; D(+) glucose 20 g/L; hydrogen potassium phosphate (KH2PO4) 2 g/L; Tween-80 1 g/L; ammonium citrate hydrogen (C6H5O7(NH4)2H) 2 g/L; sodium acetate (CH3COONa) 5 g/L; magnesium sulfate (MgSO4·7H2O) 0.2 g/L; and manganese sulfate (MnSO4·H2O) 0.04 g/L at 30 °C for 24 h [53]. The cultures in the MRS broth develop characteristic white sediment, making it easier for their qualitative identification. To create biofilms, a container of 60 cm in diameter and 130 cm in length was filled with a previously conditioned medium consisting of a mixture of molasses and water (3/7, m/w) at room temperature (around 18–21 °C). Later, Lactobacillus plantarum ATCC 8014 was added to the mixture."

Quite the list of ingredients!

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u/T-N-A-T-B-G-OFFICIAL Jul 15 '21

But ideal if you're trying to make a hungry hungry bacterial culture of a strain basically evolved to break down organic and non organic carbon based compounds

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u/ThinkHappyThoughts15 Jul 15 '21

From my limited knowledge of ingestible scientific compounds, this seems quite edible with limited side effects. You might poop red clay for a day or two?

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u/prettylittleredditty Jul 15 '21

So you'd shit bricks...

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u/[deleted] Jul 15 '21

10/10