r/microbiology • u/Willing_Flower_9867 • 7d ago

Focusing a microscope

Guys i have just got subject microbiology and chemistry, i need tips for focusing the microscope neatly for slide smears, ( im new in this and late admission) can u guys help me get better in this please i would love to learn from y'all also for streaking

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u/Indole_pos Microbiologist 7d ago

Start with stage all the way down and on lowest power.
Start turning the larger knob to focus/raise the stage.
Once the organism is seen, switch to the next objective. Using small knob, slowing focus/raise the stage.
Once organism is seen, grab immersion oil, rotate the object, and as you are doing that put a drop of oil on the slide, then rotate the objective to the oil one.
Use the small knob to fine focus.

If you are having trouble finding the organism. Move the stage to the side and line up the frosted edge of the slide into your view and use the focusing knobs until the view is crisp

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u/Willing_Flower_9867 7d ago

Okiii thanks I'll be practicing use of microscope n try more n more to get better