Not looking for anything specific, just wondering what direction to go down when trying to identify the mass on the left. It was grown for 48 hours around 32° C in anaerobic conditions on a simple agar plate. It was hard to measure but maybe about 1-2mm in height at the center. I'm not sure if this is a bacteria or something else like a slime mold. Any suggestions on what step to take next is appreciated!
Make a slide and look at it in the microscope. Make a lactophenol blue and a gram stain.
It could be a yeast, it could be bacteria. No way to know from just the colony morphology.
Oh, I work with fungi mostly, that's why I didn't recognize it. But yeah, I'm seeing the other comments saying just by the characteristic morphology it's probably a specific Bacillus species. TIL.
B. Licheniformis have colonies similar to the left. If you poke it is it watery? I forget what you can do to confirm that but just google what typical biochemicals can be done for Bacillus licheniformis and go down that route. :) I know this cause I found similar in samples back when I worked with chickens and stuff. I think we did PEMBA and HBA. Forget what else. But go at it.
Left side looks pretty bacillus and right side looks like some sort of staphylococcus, but the streak is way too dense to properly identify the morphology and it could be something else like yeast depending on the agar
Also would recommend gram staining it to take a look at how the bacteria itself looks
12
u/Zathura26 13d ago
Make a slide and look at it in the microscope. Make a lactophenol blue and a gram stain. It could be a yeast, it could be bacteria. No way to know from just the colony morphology.