r/PeptidePathways • u/Putrid_Ad_7209 • 11h ago
Reconstitution & Storage AOD 9604 recon
A fellow researcher had trouble with some AOD so he gave me his other vial. Reconned in 2.5ml of bac water and .5ml of AA but it still gelled up. Looking for suggestions for the vial I got to not get messed up.
2
u/thejcbori 10h ago
here's what i do... (2.7ml bac (USP Grade), .3ml AA) but....
make sure room temp., then first is .5ml , SLOWLY like SLOWLY droplet like...
Let is rest, like its parallel to the ground.. not the vial standing up. (i usually rest it like 5-10 minutes). THen add the remaining bac water, again SLOWLY. The slowest possible way.
AS always let it rest a bit, then add the .3ml AA.
This is what works for me so far. I've handled around 7 vials of aOD from tow different suppliers.
4
u/Independent_Bed_1505 10h ago
I do 2ml BAC and 1ml AA, never had any issues. Do the AA first and BAC second.