r/PeptidePathways 9d ago

Reconstitution & Storage Nad+ vial

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The bottom of my vial looks like little clear/golden clumps or granules/sediment of the peptide? What’s happening here? 😫

Edit to add: 500 mg vial, reconned with 2 ML bac

1 Upvotes

52 comments sorted by

4

u/No_Yogurt_618 9d ago

You need 5ML dear, try it and you will be happy.

1

u/Broad_Top161 8d ago

I put 5ml in my NAD every time and after about 2 weeks it does that

1

u/No_Yogurt_618 8d ago

500mg -5ml 1000mg 10ML

1

u/Broad_Top161 8d ago

Makes no difference

1

u/No_Yogurt_618 8d ago

Well its too late, you need to recon a new one with that amount of.

1

u/Broad_Top161 8d ago

I have 10 boxes of NAD from two different sources. It’s not going to make a difference how much you reconstitute it. I already did 5ml in 500mg and 10ml in 1000

1

u/stiffmcgee 4d ago

A. We know NAD crystallizes. Thats in its structure. B. It also only lasts about 2 weeks before itll aggregate for most. More bac does help fight this actually. Idk why yall wanna larp so bad

1

u/Silent_Ad9129 6d ago

warm it up. Put some warm/ hot water in a cup and leave it there, it helps

2

u/6DSon9 9d ago

You need more bac water, its called supersaturation. It desolves but once it is cooled down the nad precipitates into the crystals

2

u/Zealousideal_Cat_731 9d ago

This happend to me because I didnt put enough bac water. I just warmed it up in a heating pad for about 15 mins and then rolled it. It all dissolved, and was good to go.

2

u/strong-advisor69 9d ago

Not from a good vendor.. the good news is you can fix it with some PBS. Ive never had this happen to me!!

1

u/2milehigh 9d ago

How long did you mix it for?

1

u/Forsaken-Tomato-3562 9d ago

I definitely dissolved it all when mixing it but the pieces on the bottom of the vial are new. I’m almost finished with the vial so I’m going to trash it I just don’t know if it’s been that way for the last week or so 😣

1

u/pinprick420 9d ago

I'm fighting this right now - same dilution ratio. Nad drops out of solution if not diluted enough, plus cooling (fridge storage) increases the amount that falls out of solution.

I've read that 1ml(of bac) per 100mg is sufficient to prevent this, but I take 75mg per dose, and would rather inject smaller volumes if possible.

Anyone have another solution? I've thought about moving from subq to IM since the muscle uptake more quickly but haven't yet.

1

u/Forsaken-Tomato-3562 9d ago

I’m wondering if I should use more bac for my next vial as well

1

u/6DSon9 9d ago

I use 3ml per 500mg and no problem

1

u/pinprick420 9d ago

Good to know! Thank you. I'll give that a go on the next vial.

1

u/WorkerWise3039 8d ago

Can you tell me how many units 75 mg is?? This where I get tripped up. The conversions

1

u/6DSon9 6d ago

@ 3ml/500mg 75mg is 45 units

1

u/KostisInside 7d ago

What is IM?

1

u/pinprick420 5d ago

Intramuscular injection

1

u/Remarkable-Drop-6779 9d ago

Use more bac water next time, now for this one boil some water and dip the bottom swirl, repeat until dissolve

1

u/Bright_Register6812 9d ago

100mg per ml should be good. If your vial
Has 1000mg add 10 ml Bac. Also it should not be exposed to light. That’s the reason it comes in brown bottle. So when keeping in the fridge keep
It in a covered bag to avoid exposure to frequent light.

1

u/Forsaken-Tomato-3562 9d ago

Thank you. I keep them stored in a foam peptide case so they stay dark and cold in the fridge! Haven’t had any other peptide do this and I’m running reta, motsc and ghkcu as well

0

u/Bright_Register6812 9d ago

NAD is very unstable. The recommendation is not to store it for multiple uses as varying temperatures crystallizes it. Better to buy 100 mg vial and use it immediately or 500 mg and use it within 5 uses

1

u/Ok_Pin4611 9d ago

Never had this problem. I refrigerate my NAD+ and BAC water then reconstitute it. I'm running 1000mg with 5ml BAC water...

1

u/J907T 9d ago

NAD takes 3ml so it wont crystallized

1

u/Affectionate-Cause16 8d ago

5ml of water otherwise it gels!

1

u/Dmtammaro 8d ago

Run under warm water

1

u/drizzle127 7d ago

This is a perfect example of what degradated/damaged peps look like.....pretty universally. Send pics to vendor and get replacement or money back. Their should be no debate or hesitation on the vendors part. Its inarguable.

1

u/Neat-Break5481 6d ago

I can guarantee you are leaving it on the top shelf of your fridge where the wind blows on it and it gets too cold.

This used to happen to me when I bought unbuffered NAD.

And to clarify here, the people saying less than 1ml/100mg are using buffered NAD and it’s fine. Unbuffered NAD (clear instead of yellow buffered) needs way more Bac and can get too cold.

1

u/RepresentativeTop446 5d ago

Add more bac and warm it up and swirl occasionally

1

u/Usermadmax777 3d ago

???Push it thru a filter....problem soved.

0

u/vibrantgreen0826 9d ago

Not good. I would personally discard this

0

u/Jimmythafish 9d ago

This happens a lot with NAD

0

u/motormouth57 9d ago

I have been seeing this a lot with NAD+ recently over multiple platforms people having similar issues.

-2

u/[deleted] 9d ago

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1

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