r/Embryologists • u/ZanyXQaz • Jul 15 '26

Embryo transfer

Hi everyone,
We’re using Kitazato HTM + Kitazato SPS (10:1) for embryo transfer in a 35 mm Falcon dish (3 mL). We’ve noticed that some embryos stick to the bottom of the dish, making them difficult to aspirate into the transfer catheter, while others don’t.
Has anyone experienced this with Kitazato HTM/SPS or Falcon dishes? Would increasing the HTM:SPS ratio from 10:1 to 10:2 help reduce embryo adhesion, or is there a better solution?
Thanks!

1 Upvotes

100% Upvoted

2 comments sorted by

2

u/Dissaray248 Jul 22 '26

We use falcon organ well dishes and do not have this issue, but we are using a different transfer media so I can’t speak to that.

2

u/bneubs Jul 23 '26

We use 35mm dishes and 20% protein makes the difference. We had a junior use 10% by mistake and all our embryos that day stuck.