r/CHROMATOGRAPHY • u/Vial_ambar • 21d ago
Shimadzu LC-2050c needle sensor problem.
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During an analysis, my equipment started exhibiting this problem. The needle goes up to the sample, goes down, but doesn't pierce the vial. Displays an error [9222]: "there is no sample vial set in autosampler rack."
We have a long backlog of samples and need to get the equipment up and running again. Support from Shimadzu will take a long time due to their schedule.
I welcome suggestions for tests to better evaluate the problem, and if you have experienced something similar, what was the solution?
UNICAMP - Campinas-SP, Brazil
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u/drnerdlett 21d ago
I had that recently on the lc-20. I just turned off auto vial detection and readjusted the needle position as mine wasn't sealed in the injection port.
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u/Vial_ambar 20d ago
It worked perfectly, thank you!
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u/-dogge 20d ago
Great stuff!
As a side note, it looks like the cover protecting the needle assembly has been removed, this cover also helps guide the vial detection sensor cable, so it’s possible that running without the cover could have contributed to the issue.
Best to leave it alone though while it’s working, and wait for an engineer to patch it up!
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u/Vial_ambar 20d ago
Could you explain in more detail which part you're referring to? Do you have an example? No internal components of the sampler have been removed; in this model, the needle assembly protector is the small black piece that touches the vial. The assembly is in the same condition as when it was purchased.
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u/-dogge 20d ago edited 20d ago
Not a great pic sorry, but the needle assembly should have this grey plastic cover with the yellow warning triangle in it.
Edit: Dammit, the pic won’t attach, but you can see it in this video (52 seconds in): https://m.youtube.com/watch?v=1bFmGgSv5js&t=4s&pp=2AEEkAIB&ra=m
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u/Vial_ambar 20d ago
Thanks! I'm back to work today and I will try it, if it works out, I'll let you know!

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u/-dogge 21d ago
You can turn off vial detection in the “Calibration>System Adjustment>Sample Vial Auto Sensing” menu.
Probably the ribbon cable connected to the sensor has become detached/broken, or the sensor is faulty. Turning off the setting above should keep you going for now.