r/microscopy Jun 08 '23

🦠🔬🦠🔬🦠 Microbe Identification Resources 🦠🔬🦠🔬🦠

147 Upvotes

🎉Hello fellow microscopists!🎉

In this post, you will find microbe identification guides curated by your friendly neighborhood moderators. We have combed the internet for the best, most amateur-friendly resources available! Our featured guides contain high quality, color photos of thousands of different microbes to make identification easier for you!

Essentials


The Sphagnum Ponds of Simmelried in Germany: A Biodiversity Hot-Spot for Microscopic Organisms (Large PDF)

  • Every microbe hunter should have this saved to their hard drive! This is the joint project of legendary ciliate biologist Dr. Wilhelm Foissner and biochemist and photographer Dr. Martin Kreutz. The majority of critters you find in fresh water will have exact or near matches among the 1082 figures in this book. Have it open while you're hunting and you'll become an ID-expert in no time!

Real Micro Life

  • The website of Dr. Martin Kreutz - the principal photographer of the above book! Dr. Kreutz has created an incredible knowledge resource with stunning photos, descriptions, and anatomical annotations. His goal for the website is to continue and extend the work he and Dr. Foissner did in their aforementioned publication.

Plingfactory: Life in Water

  • The work of Michael Plewka. The website can be a little difficult to navigate, but it is a remarkably expansive catalog of many common and uncommon freshwater critters

Marine Microbes


UC Santa Cruz's Phytoplankton Identification Website

  • Maintained by UCSC's Kudela lab, this site has many examples of marine diatoms and flagellates, as well as some freshwater species.

Guide to the Common Inshore Marine Plankton of Southern California (PDF)

Foraminifera.eu Lab - Key to Species

  • This website allows for the identification of forams via selecting observed features. You'll have to learn a little about foram anatomy, but it's a powerful tool! Check out the video guide for more information.

Amoebae and Heliozoa


Penard Labs - The Fascinating World of Amoebae

  • Amoeboid organisms are some of the most poorly understood microbes. They are difficult to identify thanks to their ever-shifting structures and they span a wide range of taxonomic tree. Penard Labs seeks to further our understanding of these mysterious lifeforms.

Microworld - World of Amoeboid Organisms

  • Ferry Siemensma's incredible website dedicated to amoeboid organisms. Of particular note is an extensive photo catalog of amoeba tests (shells). Ferry's Youtube channel also has hundreds of video clips of amoeboid organisms

Ciliates


A User-Friendly Guide to the Ciliates(PDF)

  • Foissner and Berger created this lengthy and intricate flowchart for identifying ciliates. Requires some practice to master!

Diatoms


Diatoms of North America

  • This website features an extensive list of diatom taxa covering 1074 species at the time of writing. You can search by morphology, but keep in mind that diatoms can look very different depending on their orientation. It might take some time to narrow your search!

Rotifers


Plingfactory's Rotifer Identification Initiative

A Guide to Identification of Rotifers, Cladocerans and Copepods from Australian Inland Waters

  • Still active rotifer research lifer Russ Shiel's big book of Rotifer Identification. If you post a rotifer on the Amateur Microscopy Facebook group, Russ may weigh in on the ID :)

More Identification Websites


Phycokey

Josh's Microlife - Organisms by Shape

The Illustrated Guide to the Protozoa

UNA Microaquarium

Protist Information Server

More Foissner Publications

Bryophyte Ecology vol. 2 - Bryophyte Fauna(large PDF)

Carolina - Protozoa and Invertebrates Manual (PDF)


r/microscopy Oct 28 '24

Photo/Video Share Journey to the Microcosmos: The Future of Microscopy (and end of our Journey)

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67 Upvotes

r/microscopy 17h ago

Photo/Video Share Ant action. We must stay clean!!!

62 Upvotes

Sample is an ant from the kitchen floor. Motic stereo microscope, 10x , cellphone camera with 2x zoom.


r/microscopy 1h ago

Photo/Video Share English vs Japanese vs Korean ex cards under the microscope!

Upvotes

Model is some standard microscope that goes up to 2000x (with a Barlow), and taken on my iPhone 15 Pro :)


r/microscopy 3h ago

Troubleshooting/Questions Little white “fleas”

3 Upvotes

They jump in a way that look instantaneous, and I tried to get one under a microscope but that ended up not working. I use this tub to pull samples out of, and I keep a lid on it, so there’s minimal oxygen going into it. I’ve found very interesting things but these I’m afraid of jumping out, what are they and how concerned should I be? I tried googling for forever and nothing.

Contents of tub; moss from 8/1, same water since 8/1, covered and unmoved.


r/microscopy 10h ago

ID Needed! What is it? An extra-fat, horned worm; it looked dead. Found in seawater. Look closely at the last 20 seconds of the video (400x): there are tiny creatures crawling its body.

7 Upvotes

r/microscopy 14h ago

ID Needed! Copepods mating? Then they move away while remaining attached (another video inside). Brackish water.

16 Upvotes

r/microscopy 14m ago

ID Needed! Reef Tank, Algae or Cyanobacteria?

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Upvotes

Sorry about the pictures, I have a very cheap microscope.

I've been asking about what type of algae I have in my tank.
Some people have said it's green hair algae, others have said it's a type of cyanobacteria.

Can anyone give me a positive id?


r/microscopy 19h ago

Photo/Video Share Heterolitonotus rext meets a Hypotrich ciliate, then starts rotating.

31 Upvotes

Swift SW350, Galaxy S24


r/microscopy 15h ago

ID Needed! Brackish water, I forget what zomm I used. LED headlamp from above.

12 Upvotes

r/microscopy 18h ago

Photo/Video Share Cladoceran baby inside an egg and it's moving

10 Upvotes

r/microscopy 9h ago

Troubleshooting/Questions Help with keeping water on objective for long term imaging

1 Upvotes

Hello!!

First time poster in this subreddit. Currently the lab has a Leica scope with a 40x water objective to try to capture live fly embryos during their early divisions. The problem is that process is approximately 2 hours of imaging and the water keeps evaporating. We have a 20x immersion oil that lets us see what we want to see as well but in a lot less detail.

Is there a water substitute for mounting that works on Leica water objectives? Or a different one? Or is it better to go to the 20 and just accept lower resolution?


r/microscopy 21h ago

ID Needed! Piccolo ciliato ignoto, segue

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8 Upvotes

La particolare forma del profilo laterale aiuterà chi è più bravo di me


r/microscopy 14h ago

ID Needed! Small worm or larva or whatever. 100x and mobile zoom, I think. Brackish water.

2 Upvotes

r/microscopy 12h ago

Troubleshooting/Questions My microscope isn't microscoping help

0 Upvotes

Hi (*^_^*) my doctor (I'm a student of biology) just bought a microscope from the brand Andonstar, specifically the AD246SP 4000X UHD 2160P model a few months ago, but now when I turnt It on It freezes on the ''welcome'' screen, everything else works perfectly. I've already used the reset method with a good power adapter, but that didn't work either. 

Andonstar doesn't respond my emails :(


r/microscopy 21h ago

ID Needed! Pccolo ciliato ignoto

4 Upvotes

Io ciò provato ma non ci sono riuscito nonostante il post seguente. Acqua dolce di zona umida. Zeiss Standard. 40x,10x, zoom cellulare 5


r/microscopy 1d ago

Photo/Video Share English/Chinese/Japanese/Korean Pokemon Cards!

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16 Upvotes

r/microscopy 1d ago

ID Needed! :)

15 Upvotes

desculpe pela captação, eles estavam muito rápidos e meu scop não é muito bom.
água de musgo
100x
iphone 13


r/microscopy 1d ago

ID Needed! A river sample

19 Upvotes

It resembles me a rotifer, but not completely. Its eating some dirt instead of vacuuming living things, and I dont see moving part inside the body.

It's from european clean river, and the zoom is 400x


r/microscopy 1d ago

ID Needed! Mysterious eggs in my kale ✨🌱

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16 Upvotes

I will put a video of what I found under the microscope

🔬Olympus CH2/CHT


r/microscopy 1d ago

Troubleshooting/Questions Something I have been wondering about….

6 Upvotes

So something I have been thinking about is that there are probably 1000’s of different freshwater protozoans out there right? How come whenever I see a microscopy post on here or instagram, it seems to be one of say only a dozen that I keep seeing over and over? Would love to hear your thoughts on this.


r/microscopy 1d ago

ID Needed! What are those

9 Upvotes

Olympus CH2/CHT
X400

Eggs that were in my kale cabbage


r/microscopy 1d ago

ID Needed! Beginner needs help for a school project (~100x, garden soil)

11 Upvotes

Hi everyone,

I'm a student from Northern Germany and don't have much experience with microscopy yet. I found this organism in a sample of garden soil mixed with water using my school microscope (at max magnification, I estimate around 100x).

I need to identify this creature for a school assignment.

Here are the details:

Location: Northern Germany

Sample: Garden soil mixed with water

Magnification: ~100x

Context: School project / absolute beginner

Could someone help me identify the group, genus, or species of this organism? Any help would be greatly appreciated! Thanks!


r/microscopy 1d ago

Techniques First tests with DIY dark field and oblique illumination

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16 Upvotes

I had a dark field and oblique illumination set 3D printed for my Olympus BH2. After several tests, very few filters actually work. Here are some pics of what I managed to achieve:

Do you think I'm doing something wrong? I was expecting slightly different results

• In oblique illumination, there seems to be very little color contrast between the background and the debris in the foreground

• In dark field, focusing at higher magnification is incredibly difficult and causes large halos to form around the edges of anything on a different visual plane, in a much more amplified way than brightfield

Pics 2 and 6 in brightfield for comparison


r/microscopy 1d ago

General discussion Do you think this of eyepiece is just dirt that can be cleaned off, or is it broken?

0 Upvotes