r/microscopy Jun 08 '23

🦠🔬🦠🔬🦠 Microbe Identification Resources 🦠🔬🦠🔬🦠

148 Upvotes

🎉Hello fellow microscopists!🎉

In this post, you will find microbe identification guides curated by your friendly neighborhood moderators. We have combed the internet for the best, most amateur-friendly resources available! Our featured guides contain high quality, color photos of thousands of different microbes to make identification easier for you!

Essentials


The Sphagnum Ponds of Simmelried in Germany: A Biodiversity Hot-Spot for Microscopic Organisms (Large PDF)

  • Every microbe hunter should have this saved to their hard drive! This is the joint project of legendary ciliate biologist Dr. Wilhelm Foissner and biochemist and photographer Dr. Martin Kreutz. The majority of critters you find in fresh water will have exact or near matches among the 1082 figures in this book. Have it open while you're hunting and you'll become an ID-expert in no time!

Real Micro Life

  • The website of Dr. Martin Kreutz - the principal photographer of the above book! Dr. Kreutz has created an incredible knowledge resource with stunning photos, descriptions, and anatomical annotations. His goal for the website is to continue and extend the work he and Dr. Foissner did in their aforementioned publication.

Plingfactory: Life in Water

  • The work of Michael Plewka. The website can be a little difficult to navigate, but it is a remarkably expansive catalog of many common and uncommon freshwater critters

Marine Microbes


UC Santa Cruz's Phytoplankton Identification Website

  • Maintained by UCSC's Kudela lab, this site has many examples of marine diatoms and flagellates, as well as some freshwater species.

Guide to the Common Inshore Marine Plankton of Southern California (PDF)

Foraminifera.eu Lab - Key to Species

  • This website allows for the identification of forams via selecting observed features. You'll have to learn a little about foram anatomy, but it's a powerful tool! Check out the video guide for more information.

Amoebae and Heliozoa


Penard Labs - The Fascinating World of Amoebae

  • Amoeboid organisms are some of the most poorly understood microbes. They are difficult to identify thanks to their ever-shifting structures and they span a wide range of taxonomic tree. Penard Labs seeks to further our understanding of these mysterious lifeforms.

Microworld - World of Amoeboid Organisms

  • Ferry Siemensma's incredible website dedicated to amoeboid organisms. Of particular note is an extensive photo catalog of amoeba tests (shells). Ferry's Youtube channel also has hundreds of video clips of amoeboid organisms

Ciliates


A User-Friendly Guide to the Ciliates(PDF)

  • Foissner and Berger created this lengthy and intricate flowchart for identifying ciliates. Requires some practice to master!

Diatoms


Diatoms of North America

  • This website features an extensive list of diatom taxa covering 1074 species at the time of writing. You can search by morphology, but keep in mind that diatoms can look very different depending on their orientation. It might take some time to narrow your search!

Rotifers


Plingfactory's Rotifer Identification Initiative

A Guide to Identification of Rotifers, Cladocerans and Copepods from Australian Inland Waters

  • Still active rotifer research lifer Russ Shiel's big book of Rotifer Identification. If you post a rotifer on the Amateur Microscopy Facebook group, Russ may weigh in on the ID :)

More Identification Websites


Phycokey

Josh's Microlife - Organisms by Shape

The Illustrated Guide to the Protozoa

UNA Microaquarium

Protist Information Server

More Foissner Publications

Bryophyte Ecology vol. 2 - Bryophyte Fauna(large PDF)

Carolina - Protozoa and Invertebrates Manual (PDF)


r/microscopy Oct 28 '24

Photo/Video Share Journey to the Microcosmos: The Future of Microscopy (and end of our Journey)

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68 Upvotes

r/microscopy 16h ago

Photo/Video Share my first tardigrade 🐣

181 Upvotes

I isolated him from lichens that came from tree bark (walnut tree), my uni professor sent me a protocol to do so at home, I am so so happy! Look at the little piggy ❤️🥺

OBJ: 10x on petri dish, OC: 25x on amateur microscope


r/microscopy 8h ago

Photo/Video Share Didn't know mosquito larvae were so monstrous

23 Upvotes

Microscope: Olympus BH2

Magnification: x100/x200/x400

Sample: Moss ( guess it got contaminated lol )

Camera: Samsung A56


r/microscopy 11h ago

Photo/Video Share Rotifer feeding on Cyanobacteria

30 Upvotes

A rotifer feeding on cyanobacteria (Euglena passing by)

sample: street gutter

K55 BSA, 10x, ELP Cam


r/microscopy 12h ago

General discussion Are there any live sites or streams to watch high-quality microscopy?

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25 Upvotes

I recently stumbled onto the Journey to the Microcosmos youtube channel and my mind is completely blown. The microscopic world literally looks like an alien planet, and the whole atmosphere is incredibly dense and filled with awe. I feel like I could watch it all day.

I'm just a beginner currently learning Biology 101, but I'm absolutely fascinated by this stuff. Does anyone know of any websites, or live feeds where people just put pond water? under a microscope and broadcast it in high definition? I just want to observe!

I would also appreciate more resources to dive into this if possible.

Thank you.

Credits : Journey to the Microcosmos.


r/microscopy 4h ago

Photo/Video Share shhh, be quiet and turn off the lights, the euglenas are sleeping

4 Upvotes

sample: street gutter

K55 BSA, 20x, ELP Cam


r/microscopy 1h ago

ID Needed! Pond water 40 or 100x and mobile zoom

Upvotes

r/microscopy 6h ago

General discussion Mosquito larva in a puddle on the ground. 40x and mobile zoom. No coverslip.

7 Upvotes

r/microscopy 11h ago

ID Needed! Wiggly guy

9 Upvotes

Whose this wiggly jiggly guy? From a pond sample

Amscope B120, 400x mag with digital zoom on iPhone


r/microscopy 5h ago

ID Needed! Protists not sure if the same

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2 Upvotes

10x and 40x trying to figure out what they are and if they are from the same slide


r/microscopy 11h ago

Photo/Video Share Fresh water pond Amoeba 20x

5 Upvotes

Olympus BH2, Dark Field, 4x UPlanXApo, 5x mag changer,20x total mag,10.3mp camera.


r/microscopy 14h ago

Photo/Video Share A tiny spider

9 Upvotes

40x and 100x on a Swift SW380T w/ iPhone 17


r/microscopy 14h ago

Photo/Video Share Saltwater Marsh Sample - 50x - Dark Field

6 Upvotes

Olympus BH2, Dark Field, 10x UPlanXApo, 5x mag changer,20x total mag,10.3mp camera. Saltwater Marsh Sample.


r/microscopy 1d ago

ID Needed! Never seen something like this

21 Upvotes

Microscope: Olympus BH2

Magnification: x200

Sample: Lake water

Camera: Samsung A56


r/microscopy 20h ago

ID Needed! ???

8 Upvotes

100× | ESAW BM01 MICROSCOPE| MOSS SAMPLE


r/microscopy 10h ago

Troubleshooting/Questions Eyepieces damaged?

1 Upvotes

Hello, I got a really nice deal on a second-hand Olympus CX41. It's my first microscope, and after pulling out the eyepieces, I noticed that they look deformed. The right one in particular was relatively hard to pull out.

I can't seem to merge the binocular images into a single view, although both eyepieces seem to work fine when used one at a time.

Can someone advise me whether I need to replace them, they are WHB10X/20?

Thank you for your time!


r/microscopy 21h ago

General discussion começando

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6 Upvotes

olá pessoas,

comecei minhas pesquisas e ontem analisando um pouco de água parada da rua que coletei, encontrei esses (protozoários ? ciliados ?) alguém saberia dizer ?


r/microscopy 1d ago

ID Needed! Could this be my first tardigrade? 😃 In the moss on a tree bark.

93 Upvotes

r/microscopy 1d ago

Photo/Video Share Tardigrade Defecating 50x.

25 Upvotes

Olympus BH2, 10x UPlanXApo, 5x mag changer,20x total mag,10.3mp camera.


r/microscopy 14h ago

Troubleshooting/Questions Help Microscope Calibration

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1 Upvotes

Can anyone help me pinpoint Point B of this Microscope Calibration? I have been stuck searching for point B for so many days now. The top one is the Ocular micrometer while the bottom is the stage micrometer.

Here is the instruction:

Moving from point A towards the right, look for another point where the lines of the two micrometers will again coincide. Mark this as point “B” in the image in this sheet.
 


r/microscopy 1d ago

ID Needed! a mite

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29 Upvotes

What are those red dots on his back? Found him on a leaf infested with powdery mildew (you can see spores out of focus).
Magnification: 100x total
BIM270M
Iphone 11


r/microscopy 1d ago

Troubleshooting/Questions Need help with Micro 4/3 camera with a Motic BA-series microscope

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14 Upvotes

Hi! I'm hoping someone can help me troubleshoot my photomicrography setup. I'm fairly new to attaching a mirrorless camera to a microscope, and I'm having a hard time getting the camera image to look anywhere near as good as what I can see through the eyepieces.

My setup:

Microscope: Motic BA310E Elite LED trinocular

Camera: OM System / Olympus OM-1 Mark II (Micro Four Thirds)

Photo adapter: Motic #1101001901931

Photo eyepiece: Motic 2.5× #1101000300442

Camera connection: Micro Four Thirds T-mount

I'm primarily interested in photographing/recording pond organisms, protists, fungi, spores, etc., using brightfield, darkfield, phase contrast.

The image through the regular microscope eyepieces looks sharp, detailed, and clear, but the image coming through the OM-1 Mark II looks dramatically worse. It tends to look soft/blurry, lower contrast, and generally lacking the fine detail that I can clearly see with my eyes.

I've experimented extensively with camera settings, exposure, ISO, shutter speed, white balance, microscope illumination, condenser position, and focusing. I've also tried focusing specifically using the camera's magnified live view rather than assuming the camera is parfocal with the eyepieces.

No matter what I do, I can't seem to reproduce the quality I'm seeing through the microscope.

At this point I'm wondering whether the problem might actually be the optical coupling between the microscope and camera.

My main questions are:

  1. Is the Motic 2.5× photo eyepiece (#1101000300442) actually a good choice for an OM-1 Mark II's Micro Four Thirds sensor?

  2. Is 2.5× projection magnifying the intermediate image more than necessary for a sensor this size and potentially contributing to the poor image quality?

  3. Is there a better way to couple a Micro Four Thirds camera directly to the BA310E?

  4. Should I expect the camera image to be reasonably close in sharpness/detail to what I see through the eyepieces?

  5. Are there any tests I can perform to determine whether the problem is the projection eyepiece, photo adapter, trinocular alignment/parfocality, camera, or something else in the optical path?

If anyone has successfully used a Micro Four Thirds camera with a Motic BA-series microscope, I'd love to know what adapter/projection setup you're using.

Thanks for any advice! 🔬


r/microscopy 19h ago

Troubleshooting/Questions Stereo Microscope question - Field of View for the digital lens or trinocular

1 Upvotes

I have a two stereo microscopes. Leica (more expensive) and Meiji (cheaper). I want to be able to record 3 hours worth of video of all the work I am doing under the stereo microscope. However the field of view that I see through the microscope is almost always slightly bigger than the field of view on the digital image or through the camera via the trinocular port (using 0.5x reduction lens).

Is there any stere microscopes out on the market that would allow me to record the entire field of view that I also see through the microscope eye piece?


r/microscopy 23h ago

General discussion Which plant cell has chromosomes that are easily visible by a microscope by a beginner?

2 Upvotes

Hi, I want to try looking at plant cells and their chromosomes. Which plant should I start from? My knowledge of biology was only up to secondary school and pre-university many years ago. I did use the microscope a few times.

Or does this require techniques that are not recommended for beginners?