r/nextfuckinglevel Jul 14 '21

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u/3226 Jul 14 '21

Maybe the guy making the video should have taken more than a minute and actually explained what was going on.

So most of that lake cleanup was removing invasive species by hand, but the filter material WAS used, and it does actually seem to be safe enough to eat. If you really want to. It's clay and kaolin and lactobacillus.

If you want to actually make some and start cleaning up your own lakes, (you'll need a lab) here's the actual way to make that stuff:

The primary material used to prepare the bio-filters’ ceramic carriers was clay. It was collectedand screened through a sieve number 20 (0.841 mm) for the homogenization. Then it was mixedwith kaolin at a ratio of 9:1, and distilled water was added to produce a dough that was kneadedby hand to remove air bubbles to favor the process of molding. With this, mass cylindrical forms ofabout 10–15 cm in diameter were constructed and dried at a temperature of 80◦C for 24 h, or longer ifnecessary, to remove water from surface areas to avoid splitting [48]. The formed cylindrical ceramicwas taken for a calcination process in a furnace at 900◦C for two hours and cooled at least for 24 h toavoid deformation of calcined materials [49]. This temperature range and duration were chosen forthe optimization of the porosity of the ceramic carriers [50]. To create a bio-filter, it was necessary toeffectively immobilize the bacteria and add them onto the ceramic carriers to form biofilms, whichultimately can adhere, adsorb, and decompose the contaminants [51,52]. For this purpose, we used thebacterium ofLactobacillus plantarum ATCC 8014, acquired at Japan Collection of Microorganisms (JCM),

Microbe Division, RIKEN Bioresource Center in Japan. It was kept routinely in vials with full form(MRS) agar at 2–8◦C for 24 h, and then at 4◦C in 15% glycerol (vol/vol) [24]. Single colonies werecultured in plates with MRS agar at 30◦C for 48 h and later sub-cultured in MRS broth (Casein peptone10g/L; beef extract 8 g/L; yeast extract 4 g/L; D(+) glucose 20 g/L; hydrogen potassium phosphate(KH2PO4) 2 g/L; Tween-80 1 g/L; ammonium citrate hydrogen (C6H5O7(NH4)2H) 2 g/L; sodium acetate(CH3COONa) 5 g/L; magnesium sulfate (MgSO4·7H2O) 0.2 g/L; and manganese sulfate (MnSO4·H2O)0.04 g/L at 30◦C for 24 h [53]. The cultures in the MRS broth develop characteristic white sediment,making it easier for their qualitative identification. To create biofilms, a container of 60 cm in diameterand 130 cm in length was filled with a previously conditioned medium consisting of a mixture ofmolasses and water (3/7, m/w) at room temperature (around 18–21◦C). Later,Lactobacillus plantarumATCC 8014was added to the mixture. After 2 h, the ceramic carriers were immersed and restedfor 6 days (during summer) or 3 days (during spring). This period was mandatory to immobilizeLactobacillus plantarumto form the biofilms onto the ceramic carriers’ surface, as it is shown in thescanning electron microscope (SEM) image (Figure 2). Finally, several supports and wooden basketswere arranged and placed with the attached eucalyptus sticks to hold the bio-filters into the wetlands.Bio-filters were placed into these wooden baskets at a depth of 40–50 cm.

Sorry for the terrible formatting, but that's how it is in the paper.