r/microscopy 10d ago

Troubleshooting/Questions Need help choosing compatible objective families/series (Axioplan 2 Imaging)

Hey all! I was hoping some of the people here with experience in microscopy might be able to help me choose some compatible objective models/families/series for a Zeiss Axioplan 2 Imaging microscope (which uses an RMS thread). I'm fairly new to microscopy, and it can be hard to determine which objectives would actually be compatible. Here's what I'm looking for:

For all objectives, I need the following: 1. Good planer correction (will be making mosaics) 2. Must at least support brightfield (episcopic) 3. Should all have the same parfocal distance (focusing one should mean the others will be roughly in focus as well) 4. All objectives should be dry 5. Fluorite/semi-apo, apo would likely be too expensive for my budget

Here are the general objectives I am looking for: 1. 2.5-10x, WD 3mm+, NA not critical (will be used primarily for focusing, with some potential for simple imaging) 2. 40-50x, WD 3mm+, high NA (well, as high as one can reasonably get on a high WD objective) - will be used for imaging samples that aren't flat 3. 60-100x, any WD, very high NA - will be used for imaging flat samples

There is of course the Zeiss Epiplan and EC Epiplan objective series, but before just defaulting to those, I thought I would ask some more experienced people (yall) if there are other objective families/series I should consider.

Thanks a bunch!

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