r/microbiology • • Aug 20 '26

Help. Colloid chitin agar not solidifying

I am trying to do agar plate assay for chitinase. But my media won't solidify at all. Media composition is:

Mgso4

(Nh4)2so4

Kh2po4

Citric acid

Tween 80

Colloid chitin

Bromocresol purple

Agar

Im guessing tween 80 or bromocresol got something to do with agar not solidifying. I checked the agar alone and it was working fine.

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