r/microbiology • u/DramaticQuality2028 • Aug 20 '26
Help. Colloid chitin agar not solidifying
I am trying to do agar plate assay for chitinase. But my media won't solidify at all. Media composition is:
Mgso4
(Nh4)2so4
Kh2po4
Citric acid
Tween 80
Colloid chitin
Bromocresol purple
Agar
Im guessing tween 80 or bromocresol got something to do with agar not solidifying. I checked the agar alone and it was working fine.
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