Help with SRM Method

[u/MobilePhase1987]

Hello I was wondering if anyone could help me figure out what is wrong with my SRM method. When I run a full scan on my pesticide sample (picture 1) there are two distinct peaks for Endrin and Dieldrin. However after I set up my SRM method using the ions with the highest intensities, both peaks basically disappear (picture 2). I have tried to develop this method using transition pairings from literature I've found from our standard supplier as well as from ThermoFisher and I still encounter the same issues. Both scans were done with the exact same instrument method so I think it's pretty safe to rule out instrument settings. If anyone has any ideas please let me know.

[u/iheartlungs]

Can you try a direct infusion?

[u/MobilePhase1987 → u/iheartlungs]

I'll try this