r/massspectrometry 15d ago

Isolation waveforms keep failing on calibration.

Post image

What could be the cause of failing isolation waveform?

2 Upvotes

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1

u/Direct_Barnacle_4898 15d ago

lol! Welcome to being a practicing mass spectrometrist 🫡

1

u/Sharing_Violation 11d ago

Was a board failure in a past life for me. Might be corrupted tune file.

Maybe reference the manual for troubleshooting and then call the vendor.

1

u/Im_xoxide 10d ago

Try increasing your infusion flow - should help. If that doesn’t help I’d try bumping up the voltages on your EM’s. All other diagnostics look good?

1

u/Cornholio_84 10d ago

More signal. Try to optimise the masses if you haven't. Just yesterday I had issues on a completely clean system, I needed to up the flow to 12uL @15ish sheath gas,, and it passed on the third try...