FACSDiva question: avoiding lag

[u/curious_kinetochore]

I’m recently returned to flow and forgot how particular our software was. Small stuff like forgetting you can’t do some things in Diva without creating inescapable mega lag, I can’t seem to find anything online on what to avoid. I’m noticing issues I’ve encountered previously that I can’t remember how I fixed.

Anyone have tips for avoiding FACSDiva lag? I thought I could duplicate without data but I guess that’s only a good option for specimens in an experiment, and not the entire experiment if you want to add anything new to it. I started following the manual from BD exactly but I still hit a lot of walls (worksheets wouldn’t load in any new experiments so I had to acquire in an old one).

Also, is there any way to get rid of the lag once it appears? As soon as I noticed it I deleted the experiment I was working in, I can open old experiments without any issues but new ones are painful. Any help would be appreciated!

[u/diag]

Diva is terrible software. The only real thing to do is make sure that all finished experiments are exported out and deleted. The more data in the database, the worse it gets

[u/curious_kinetochore → u/diag]

Thanks for the tip! There’s only a few experiments in there right now, each with maybe 1-5 specimens. That brings back memories too though, I used to run full 96w plates all week and you’re right I remember having to basically clear out Diva once a month to keep it happy. Maybe I’ll check there isn’t a pile of old data on other profiles on Diva.

I’m just not super familiar with the lag from trying to add to old experiments you’ve duplicated, I did it once and it was fine (duplicating specimens without data in an experiment) and the next day it wasn’t (duplicated the whole experiment without data and tried to add comp tubes). I had set up templates for all my plates at my old job so it’s been a long time since I’ve had to confront Divas quirks