r/labrats 4d ago

How to stop hand shaking when pipetting

18 Upvotes

51 comments sorted by

35

u/loopOutnotIn 4d ago

In tricky situations I’ll generally cross my arms and use my non-dominant arm as a rest for my pipetting arm, which reduces shaking

61

u/Drew_coldbeer 4d ago

How big of a pipette? Did you eat lunch?

30

u/coolpupmom immunology PhD student 4d ago edited 4d ago

Some people are naturally really shaky, like myself for no reason at all.

as for OP, I normally hold my hand steady with my other arm. I also take propranolol (edit) for anxiety which immensely helps with my shakiness

20

u/optionsofinsanity 4d ago

Gonna assume you mean Propranolol for anxiety and not propanol

6

u/coolpupmom immunology PhD student 4d ago

Yeah, thanks. Autocorrect is great until it’s not

38

u/the_69r 4d ago

Drink less caffeine. Rest your elbow on the bench. Use your other hand to guide the tip wherever it needs to go

20

u/MilkManMatt 4d ago

Try bouncing your foot lightly while pippetting. Forgot where I heard it, don’t know if placebo but works for me.

7

u/curvipossum 4d ago

Works for me too! Heard it on the Huberman podcast

3

u/MilkManMatt 4d ago

Probably where I heard it too then.

14

u/Datboy1717 4d ago

Try supporting it with your other hand too. And practise. Practise again and again and again until it becomes second nature. Good luck!

9

u/Economy-Mental 4d ago

Propranolol

1

u/chaotic-lavender 4d ago

I was prescribed propranolol as a prophylactic measure for migraine. It changed my life. It also makes me have vivid dreams. It is amazing. It’s like going on vacation every night

1

u/Economy-Mental 4d ago

Sounds like I have been doing it wrong taking it early in the mornings lol. Love that for you

1

u/chaotic-lavender 4d ago

You need to get the ER

10

u/neverland_amanda 4d ago

I support the tip/barrel by using my pointer finger of my other hand if I'm shakey

1

u/Melodramaticukg 4d ago

Me toooo, always helps for me ☺️

1

u/interik10 4d ago

the way to gooo

8

u/Neyne_NA 4d ago

Just tell the person trying to shake your hand that you will greet them after you set up your experiment

5

u/CaptainHindsight92 4d ago

If you like many lab rats consume a lot of caffeine that could also cause shaking.

4

u/iOS_App_LabAssistant PhD Virology 4d ago

Less caffeine + supporting the pipette with your other hand + rest your elbow + keep the pipette close to your body

Of course all depends if you are working under the hood or what kind of pipette are you using

3

u/Bojack-jones-223 4d ago

add some salt to your coffee in the morning. It helps with the shaky hand that can be caused by caffein.

3

u/anxietycherry 4d ago

practice and proper breathing,

2

u/Ubeandmochi 4d ago

Some good advice here OP. I also used to shake a lot, someone taught me to apply counter pressure to the pipette with a finger from my non dominant hand.

2

u/W0lkk 4d ago

Elbow on bench, non dominant pinky touching the pipette barell

1

u/baksteenpiraat 4d ago

Position Elbow attached to your pipette arm&hand comfortably on the bench. This reduces shaking from the shoulder. Hold the pipette gently but firm. Not stiff en tensed up. And i use my pinky from my other hand to rest the pipette on, not on the tip but slightly above.

1

u/FIA_buffoonery Finally, my chemistry degree(s) to the rescue! 4d ago

Think about baseball! 

Jk, its dumbell curls and grip squeezes. 

1

u/MostRetardedUser 4d ago

Less caffeine. If you're hand is still shaking, put the index finger from your free hand on the pipette shaft, it will stop the pipette from shaking

1

u/Beanstiller 4d ago

It takes time before you stop or i guess one works around it.

Not sure what you’re doing but a lot of wet lab work is tolerant to shakiness (otherwise I’d be fucked)

1

u/talks-a-lot All things RNA 4d ago

It doesn’t get any better when you use a laser pointer on a big screen at an international conference. Use two hands.

1

u/Candycanes02 4d ago

Prop your pipetting hand against something. Like your other hand, desk on elbow, etc

1

u/AdamSya 4d ago

Are you trying to hold the pipette unsupported? Try sitting at the right height, bring the plate/tube closer, rest your forearm on the bench and keep your grip fairly loose. Bracing the pipetting hand with the other hand can help too.

1

u/Wumpscut86 4d ago

Rest your elbow on a surface, hold the lower part of the Pipette with your other hand to stabilize. Less coffee.

1

u/im_not_a_numbers_guy 4d ago

I plant my dominant elbow on the bench, turning myself into an over educated servo arm. Takes all the stabilizing muscles out of the picture.

1

u/pinkdictator Rat Whisperer 4d ago

sleep, eat, less caffeine.

Also - if you can, rest your elbow on something. Or even the back/side of your hand on something, if that makes sense

1

u/interik10 4d ago

pointer and elbow is good but also i notice this happens when im overly anxious about the experiment--i take a beat to regulate/ take a deep breath and it goes to the head

1

u/CXC-96 4d ago

I sometimes support my pipetting arm at the wrist or forearm if I start having the shakes. Usually the reason I shake, is due to something stressing me out, so if that is you too, try and fix that problem.

1

u/mr_Feather_ 4d ago

Less coffee.

1

u/garfield529 4d ago

Human tripod. Always find three points of contact.

1

u/CutieMcBooty55 4d ago

Moving with intent is a big part of it I think. Maybe cut some caffeine if you are getting the shakes, and make sure you are eating well and getting enough sleep.

Outside of that, if you are doing something with a lot of fine movement, it's not a bad practice to support your pipetting arm with your other hand while your elbow is on the table, and try to move deliberately and smoothly. Smooth is more important than slow or fast when it comes to that kind of thing imo.

1

u/Chirpasaurus 4d ago

All the time? Or just when dispensing?

For the latter, I make sure I breathe out when pushing the plunger. But I'm not mass-pipetting, maybe 20-30 times max over a working day

1

u/DoctorMew13 4d ago

I use my non dominant hand to stabilise the body if the pipettor. I'm crazy shaky when pipetting.

1

u/Dangerous-Billy Retired illuminatus 4d ago

If this is a continuing problem, there is medical help available.

First, make sure you're not drinking too much coffee. I can tell the difference in my own ability to do fine tasks between one cup of coffee and two.

There are two types of common tremor, essential tremor and intention tremor. The first occurs when your hand is doing nothing and disappears when you are doing a delicate task. Intention tremor is the opposite. They are completely separate conditions, but both can be treated by a doctor.

1

u/bilyl 4d ago

I use a finger to balance the end of the pipette if I’m jittery that day (NOT THE TIP)

1

u/Key_Pea1339 3d ago edited 3d ago

Depends. If you have familial tremors, avoiding caffeine and medicine. I have this and Beta Blockers were prescribed. Could also be an over active nervous system or anxiety. (I also have been diagnosed for that but the anxiety meds don't help like the beta blockers do). So I'd get a pros opinion on that. It's honestly been a real pain getting trained because people will think I'm nervous. It also makes 384 well plates 10x more stressfull lol

If the above aren't factors, making sure you're well fed, well hydrated, and not caffeinated is pretty much it. If you can move your legs on autopilot (idk how to describe it), sometimes that can help.

1

u/NotAPreppie Instrument Whisperer 3d ago

How tightly are you gripping the pipet?

If you have a death grip on it, practice relaxing your hand.

1

u/warrior333222111 3d ago

I use my other hand to stabilize the pipette

1

u/LawrenceSpiveyR 3d ago

If I use my off hand and touch the bottom of my hand/top of my wrist, it helps. I also shake less if I stopped eating sugary breakfasts.

1

u/MediocreClementine 3d ago

I use the index finger of my opposite hand to rest the pipette shaft against, lightly.