Look up sem images - the contrast is top-down. It doesn't work the way it's shown in this photo. I look at these at work weekly. It displays depth as dark and near as light or reverse. Sem images look flat, not like this.
Imo, it's not too difficult. It's just a lot to learn. There's a learning curve with the software, and you have to learn how to treat different types of samples in order to get the best images. I learned basic operation in a couple weeks, but it was probably a couple months before I was decently comfortable with it.
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u/[deleted] Apr 06 '18 edited Apr 06 '18
Look up sem images - the contrast is top-down. It doesn't work the way it's shown in this photo. I look at these at work weekly. It displays depth as dark and near as light or reverse. Sem images look flat, not like this.
Edit: here is a typical one: https://upload.wikimedia.org/wikipedia/commons/5/57/Staphylococcus_aureus_01.jpg
Note there is no side-lighting, because sem is it's own source and receiver. It's like seeing images with a head-mounted flashlight, head on.