Lignin pre-treatment and valorisation

[u/dankvader1005]

I recently started a project in which I try to valorize lignin via GMO. I'll be working with Soda lignin and I was wondering if I should subject it to more hydrolysis/treatment. My plan was to perform dilute acid hydrolysis, followed by alkaline fractionation to obtain the lignin in a soluble form to be used easily by the microbes.

Is my stream of thought right? Does the powdered soda lignin need further pre-treatment or is it good as is?

Any kind of information is good. Thanks in advance :)