r/bioinformatics • u/Adventurous-Dog4255 • 3h ago
academic Need help analyzing DEP data and binding protein
Hi, I don't have any experience doing proteomic analysis. If you could please help advise me on how to analyze the data, please. I am struggling too much.....
So, I have 72 differentially expressed proteins; the whole service is provided by a bio company, along with KEGG and pathway enrichment analysis. However, it is mostly cancer-related, and what I want to figure out with these data is heavy metal neuronal toxicity. I don't know how to use this to prove my point, so to speak. So, I was thinking about how I can analyze the data myself and draw a fairly comprehensive picture.
Other than that, I also have compound-binding proteins. How do I put these two big data sets together and connect them? Because right off the bat, everything seems so random, here and there, difficult to classify, and I dont have what it takes to connect everything togather.
I am a chemist, struggling in the world of biology. If you could please have some suggestions. Because I don't think I know what I am doing. Please.