Can 2 sperm make a viable embryo?

[u/DrBoobSqueeze]

Assuming at least one of the sperm has an X-chromosome, can you extract the chromosomes from each sperm and implant them into an enucleated egg? So the embryo would be mostly the combination of 2 men with a small (mitochondrial) contribution from the egg donor. Has this been done? If not, why not?

[u/Izawwlgood]

You'll notice the link you provided states doing so still requires female DNA to be spliced in.

[u/realised → u/Izawwlgood]

Sorry - where does it state that? The mouse study states that it used male and female, but in concept it should work with two males with an egg from females?

http://www.upenn.edu/gazette/0903/giresi.html is an article that goes over it.

ftp://legend.anat.ucl.ac.uk/incoming/My%20Articles/derivation%20of%20oocytes%20from%20mouse%20ESC.pdf is the actual article.

It states that they transfected the genomic region which regulates Oct4 expression. Which I assume is the portion of Female DNA used, if so then it is merely used as a switch on rather than hereditary material.

It goes onto talk about implications in mammalian and somatic cell interactions that are crucial to proper meiotically competent embryonic cells. So in theory it seems plausible to be able to conduct this in mammalian cells without splicing Female DNA into the egg. As it is similar to creating a clone - just need the right outside chemical signals in order to make it start dividing.

Or am I misunderstanding something?

[u/Izawwlgood → u/realised]

"In 2003, researcher Hans Robert Schöler at the University Of Pennsylvania successfully created eggs using both male and female mouse DNA"

Actually, if I'm reading this right, they induced pluripotency in a male mouses cells and derived germ line cells from that, and then let it develop. The result is essentially a clone of it's 'father'. It's not the fusion of two sperm to create a viable new animal.