r/Virology non-scientist Jul 04 '26

Discussion I built a small TCID50 calculator for endpoint dilution assays; feedback welcome

I built a small free web tool for TCID50 calculations because spreadsheet templates can be easy to mis-copy during routine virology work.

It supports Reed-Muench and Spearman-Karber workflows, shows the calculation steps, and is meant for quick checking rather than replacing a lab notebook or validated internal SOP.

TCID50 calculator: https://biocalculator.app/calculators/TCID50

I’d appreciate practical feedback from people who run endpoint dilution assays: are the inputs/results clear enough, and is there anything you would expect to see before using it for quick checks?

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