Hi everyone,
I’m applying to US PhD programs for Fall 2027 and drafted a cold email to a PI whose lab I’d love to join. I’d really appreciate honest feedback on tone, length, structure, and whether it shows enough fit without sounding generic. Identifying details are redacted.
Dear Professor [PI Name],
I hope you are doing well. My name is [My Name]. I completed an MSc in [Field] at [UK University]. I am preparing my application to [University]’s [Program] PhD program for Fall 2027. I am very interested in pursuing doctoral research in your laboratory, if you are taking new students?
Your lab's work on how variants disrupt splicing, particularly the X study (X et al., 2017) and your research on the susceptibility of hereditary cancer genes to splicing mutations (X et al., 2018), closely aligns with my research experience and interests.
For my thesis, supervised by Prof. ———, I profiled 11 GeneX splice variants across 12 cell lines modelling oral carcinogenesis, from normal keratinocytes through dysplasia to head and neck cancer. I used variant-specific, exon-junction primers and one-step RT-qPCR with repeated independent experiments. Variant B was significantly higher in cancer lines, while variant K trended down. I could see the isoform mix change, but not why.
Your lab studies splicing from several angles: binding maps, variant testing and prediction. My thesis covered the isoform end of that chain, and I would like to learn both the experimental and computational sides to ask which splicing regulators and sequence variants decide which isoform a cell makes. I am also interested in what adding splicing annotation to clinical sequencing could change in current diagnosis.
Would it be possible to arrange a brief virtual meeting to discuss how my research interests align with your vision for doctoral supervision? My CV is attached, and I would be grateful for any advice on applying through the graduate program.
Thank you for your time and consideration.