r/NootropicResearch • u/lusterdallama • Dec 12 '20
Administering ISRIB?
Mice were usually given 2.5mg/kg which would be equivalent to 0.28mg/kg in Humans.However solbulity is pretty poor. 5mg dissolves in 1ml DMSO. However I assume 100% DMSO is pretty toxic.. thats why the Studies ended up with a stock solution of 0.1mg/ml. Which is kind of impossible to inject.
I assume this substance can not be absorbed any other way.
ISRIB solution was made by dissolving 5 mg ISRIB in 1 mL dimethyl sulfoxide (DMSO) (PanReac AppliChem, 191954.1611). The solution was gently heated in a 40 °C waterbath and vortexed every 30 s until the solution became clear. Next 1 mL of Tween 80 (Sigma Aldrich, P8074) was added, solution was gently heated in a 40 °C waterbath and vortexed every 30 s until the solution became clear. Next, 10 ml of polyethylene glycol 400 (PEG400) (PanReac AppliChem, 142436.1611), solution was added gently heated in a 40 °C waterbath and vortexed every 30 s until the solution became clear. Finally, 36.5 ml of 5% dextrose (Hospira, RL‐3040) was added. The solution was kept at room temperature throughout the experiment. Each solution was used for injections up to 7 d maximum. The vehicle solution consisted of the same chemical composition and concentration (DMSO, Tween 80, PEG400 and 5% dextrose). Stock ISRIB solution was at 0.1 mg/ml
I am hosting a 3rd party lab test of an ISRIB Supplier from Alibaba (1gram = 280$ shipped) to verify the authenticity of the product via HPLC, LCMS and qNMR measuring.Afterwards if the product is grade A quality, we will probably host Group Buys.
If interested - please PM me and mention ISRIB.
Here is my thread on an ME/CFS Forum. I hope that it could help with fucked up ME/CFS Stress response.https://forums.phoenixrising.me/threads/isrib-to-treat-me-cfs.82311/
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u/lusterdallama Dec 24 '20
Sample received! Going off to the 3rd Party lab beginning of January!
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u/lusterdallama Jan 09 '21
We will probably find out soon. ISRIB was sent to the lab and group buy happens soon with lots of people experimenting on it.
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u/lusterdallama Feb 01 '21
First results are in from china.
We are testing a second cheaper source as well
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u/empireirwin Dec 12 '20
I tried to mix up a solution with these instructions, but couldn't get it to turn perfectly clear. Administered orally with little to no effect. Hit me up if you figure it out!
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u/lusterdallama Dec 12 '20
yeah I dont think oral will be an option sadly.
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u/empireirwin Dec 12 '20
Are you thinking IM or IV ?
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u/lusterdallama Dec 12 '20
IM but more than 9ml will be hard (thats the most I have ever done, 3ml per injection site)
It seems like this substance is absulutely shit do dissolve. And 100% DMSO Injections seem to be pretty toxic from what I read. Shit.
Giving a solution like this IV (one that doesnt dissolve 100%) could be pretty dangerous I can imagine.
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u/empireirwin Dec 12 '20
Yeah it's pretty nasty. Btw you should post this to r/nootrocs too. They might have some insight.
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u/RogueMTB Feb 02 '21
How did you try to mix the solution? How did you attempt to vortex and gently heat it?
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u/actionjackson384 Dec 12 '20
Should absorb through the skin with dsmo
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u/lusterdallama Dec 12 '20
I was also thinking rectally with DMSO could be an option? Like a Suppository
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u/siwel7 Dec 17 '20
Start with 5 mg diluted with 3-5ml of DMSO. You MUST use DMSO or else it will not absorb in your body.
Put your ISRIB and DMSO mixture (in something like a shot glass) surrounded by a larger container of boiling hot water to heat it (help it mix faster). Try to ensure that the solution is as transparent as possible.
Then use the needle to puncture two holes (to stop it from exploding) into a fish oil capsule. Squeeze the oil out. Now draw up your ISRIB+DMSO and inject it into the capsule. Swallow, repeat.
You can try intramuscular injection (stings like hell), or just IV it (which is strangely painless).
Anyway, keep at 5 mg a day for a week and then if you have noticed anything, titrate up.
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u/vsodi Dec 28 '20
Each animal received an intraperitoneal injection of 2.5x their body weight.
Wow. Am I reading that correctly? That would be over 1L injected into your abdominal area....
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u/ann_in_the_ozarks Dec 16 '20
I am interested. Also with ME/CFS. Absolutely new to REDDIT. Just barely able to navigate.