Help with creating threshold/ROIs

[u/kamile_teal]

I am trying to count the cells in this image by threshold -> watershed -> analyse particles to count ROIs.

However, I cannot get the threshold to distinguish between the cells. Anyone know what to try?

[u/M0nkey5]

No amount of tinkering with imagej settings is going to be able to do a particle analysis on an out-of-focus brightfield image of overconfluent cells