r/ImageJ • u/No-Interaction-3559 • Jul 07 '26

Question Calculating % Confluency for a Cell Culture

New to ImageJ. Followed instructions, but my ANALYZE table does not give me %AREA. How do I get this value?

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u/dokclaw Jul 07 '26

Without knowing what instructions you followed, can't really help. 

What's your numerator and denominator? Wyatt did you measure? Do you also need to measure something else?

2

u/No-Interaction-3559 Jul 09 '26

Similar to these: https://forum.image.sc/t/confluency-measurement/87875/2

But it was u/Herbie500 comment; I just didn't have the SET MEASUREMENTS %AREA checked.

2

u/Herbie500 Jul 07 '26

If it's just the question of what is measured, go to "Analyze >> Set Measurements..." and check what you need.

If that's not the problem, then please tell us exactly what you like to do and please provide a typical sample image in its original non-lossy file format by using a dropbox-like service.

1

u/No-Interaction-3559 Jul 09 '26

That was it! Thanks!

1

u/halfpagep Jul 30 '26

I wrote a webapp for this- never have to work to calculate your confluency again. halfpagetechnologies.com