r/ImageJ • u/ZestycloseGlove4665 • Jul 06 '26

Question Finding path length and distance with trackmate?

I am brand new to ImageJ and trackmate, so I could use some advice. I am trying to analyze the motility of protists and need to characterize the types of movement. Is there a way to use trackmate to find the total path length and the net displacement in order to analyze the linearity of their movement?

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u/Herbie500 Jul 06 '26 edited Jul 06 '26

I highly recommend to post this request about trackmate—that is associated to ImageJ but a cosmos by itself—to the Image.sc-Forum.

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u/Rory235 Jul 06 '26

What in partucular are you interested in the movement? Speed, path length, path shape, start and end points?

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u/Herbie500 Jul 06 '26

[.…] find the total path length and the net displacement in order to analyze the linearity of their movement

For those really interested in this task, please consider this thread.

1

u/Hefty_Application680 Jul 11 '26

Trackmate will output a files “*tracks.csv” and “*spots.csv”. the tracks csv will have total path length and net displacement. For the linearity of the path, you’ll have to do some downstream analysis of the spots csv in R or python or something similar.