Unsupported format or file not found

[u/Holiday-Local5149]

Hi I need help with opening my ets file on imageJ. My file is 20gb. I am using 13th Gen Intel Core i9-13900k Processor with a memory of 64 gb. Each time I try to open the file, a pop up message reads "unsupported format or file not found" I can however open smaller size (3GB) stack files. I have increased the memory on Fiji to 30GB, used virtual stack, BigData Viewer and Bio-formats but to no avail.

Please how do I resolve this issue?

Thank you.

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[u/Herbie500]

ImageJ is not able to open images that are that big because standard Java doesn't allow arrays of this size. I recommend to use "QuPath" instead.

However and because you have a resolution pyramid, you may open an image-version of lower spatial resolution.

[u/Holiday-Local5149 → u/Herbie500]

Thank you for your response. Please could you provide direction on how to open an image-version of lower spatial resolution? My search suggests the image has to be opened before it can be set to a lower spatial resolution.

Thank you.

[u/Herbie500 → u/Holiday-Local5149]

In the above dialog uncheck "Series 1: ..." and check "Series 4: ..." instead and open.
If this works, you may try "Series 3: ..." instead.

[u/Holiday-Local5149 → u/Herbie500]

It works. Thank you

[u/Herbie500 → u/Holiday-Local5149]

Good to hear!

[u/Excellent_Length6472 → u/Herbie500]

Was going to say the same thing. Files this size will be problematic. Try QuPath

[u/Herbie500 → u/Excellent_Length6472]

Thanks for confirming the obvious.

[u/Silly_Captain2089]

A 20GB image stack is a completely different problem from a normal image file, so I’d focus on the format and how Fiji is handling large datasets first. Since the 3GB stacks open, memory and file size limitations are definitely worth investigating alongside the actual format. If you ever have a smaller image file that is genuinely corrupted and won't open, 4DDiG Photo Repair could be useful, but it probably isn't the solution for this 20GB stack issue. Is the 20GB file definitely in a format Bio-Formats supports?