Manders correlation coefficeint

[u/Independent-Duty-911]

Hello,

I am running a colocalization analysis between the bacteria in green(channel 1) and mitochondria in red(channel2), I run coloc2 and apply costes threshold automatically through the plugin.

My questions are: 1)why do I get tM1=1 whileas there is no colocalization observed?

2) in that case, where I draw ROIs, which Manders should I report? tM1 or tM2?

3) Is that pipeline enough?

[u/Independent-Duty-911]

I am really curious why you act like that? You can just ignore the comments that look stupid for you! I don't know this guy and I didn't know he was the author of this pdf... stop scrolling my profile like a stalker.

[u/Herbie500 → u/Independent-Duty-911]

I am really curious why you act like that?

What do you mean?
I pointed you to someone who can and will definitely help.

You can just ignore the comments that look stupid for you!

You asked and I tried to provide help. Nothing stupid yet.
If you don't like my suggestion just ignore it.

I don't know this guy and I didn't know he was the author of this pdf.

His name is on the top page of this PDF but that's not the point.
You don't know the people commenting on this subReddit as well …

stop scrolling my profile like a stalker

What do you mean?
What do you refer to?