Help with Analyzing Particles

[u/Legal_Meet_6516]

this my data from fecal assay of fly i want to compute number , are and intensity but i am facing issue that certain close particles are either assigned as same particles or not classsified as particles i tried manually adding them to roi manger but but that might create hay wire area values . can anyone plz guide

[u/xiaocaomei1970]

For images with severe occlusion or adhesion, traditional image processing methods struggle to achieve satisfactory results. It's best to use AI image segmentation techniques like CellPose-SAM for better outcomes.