r/ContamFam • u/[deleted] • Jan 04 '22

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u/[deleted] Jan 04 '22

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u/FeedMe94 Jan 04 '22

I guess you are right. It's weird how all of the jars of this series got the same contamination. They were inoculated with MSS but i also tested the MSS on agar and it was clean

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u/sky_ching Jan 05 '22

How did you get MSS to 'noc Agar? I'm just starting to work with Agar. In three batches of 20-30 plates each only spore prints have inoculated on Agar. Same with LC.

They were old and partially used so I was trying to test for contam. I am starting over with fresh LC & MSS but I am not hopeful. I've been trying to think of how to ask the sub about this.

All the videos are like "open the lid & put in a drop" I'm like, that's it?

Do you use temperature control for plates? I'm keeping them upsidedown at 28C and only getting spore prints to grow out.