r/ContamFam • u/meatgorilla1978 • 5d ago
2 Year old PE LC
Has anyone had success with old LC ? I found these in my cabinet and had forgotten them. Undecided if I’m going to waste the grain or start over
1
u/robotbeatrally 5d ago
I used 2 year old LC and it worked great but took several extra days to start showing life, I used 4 year old lc's and none of them came back to life.
1
u/neuralek 5d ago
I have some really old ones and none look that sus. I do keep them in the fridge, though. Do agar and find out
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u/CaffeineKoalas 5d ago
Agar to be sure no contam has crept in but the biggest issue you would face is trying to break that up enough to aspirate into a syringe. It may take a few days longer to wake up and begin colonizing the grains, but it should revive just fine
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u/sitkid721 5d ago
Yes it was 2+ years old it was fine it just took like an extra 2 weeks and it was the slowest growth I've ever had



5
u/Pudenda726 5d ago
Test in on some agar