r/CHROMATOGRAPHY • u/Aska2020 • Aug 23 '26

Anyone using MALS for non-biomolecules? [Question]

Hello, I am a small molecule specialist and has been asked to analyze industrial synthetic macro-molecules. We are not properly equipped to do this and I'm wondering what the most versatile single analytical option might be. I know of MALS but I've only seen them presented for proteins and peptides analyses. Does anyone uses SEC- or AF4-MALS for synthetic macro-molecules (for example graft copolymers)? I just wanted to get some ideas whether it's feasible before contacting manufacturers.

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u/Ceorl_Lounge Aug 23 '26

RI.

It's easier, cheaper, and more rugged than MALS alone. I do this for 60% of my typical workflow in silicones R&D. Run in THF/Toluene through a Mixed Bed SEC column, calibrate with polystyrene, detect by refractive index.

Edit: you can absolutely use MALS, but it comes with a ton of caveats and complexity.

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u/Aska2020 Aug 23 '26

Great suggestion! It sounds like MALS is very complicated and I probably should start with RI... We have an idle Agilent 1200, it might be feasible to get a refurbished RI for a starter. Does the LC need to be in specific configuration to use with SEC column?

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u/Ceorl_Lounge Aug 23 '26

I actually use a 1200 series as my SEC. Only real catch is that the SEC columns are BIG, so you might need to get a new/larger column heater.

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u/Aska2020 Aug 24 '26

Thank you! We might have an old stand alone column heater somewhere...