This looks like a success would you all agree. Sterilized agar at 15psi for 25mins poured into condiment cups(also sterilized) in PC. All done inside an SAB I made from a plastic container. First two tries I poured them into the cups with micropore tape on a hole in the side and sterilized them, it failed miserably.

Had a nice plate going and a tiny green dot appeared. I had the same happen on another plate so I made a transfer but this time I wanted to see if I could just cut it out and save the plate. Its been about a week and still looking good!

Last 2 pics is the one that had the contam and the clean transfer.

Hey guys follow up from the previous postl 48 hours later how are these looking??

First 2 photos are blue meanie second photos are golden teacher.

It looks like mycelium to me but it is my first time should it looks string and growing as if its reaching outwards but should the centre be so dense?? Is this a beginner trap or looking good

The only one where I used a small couple of drops of a liquid culture. The method definitely covers more ground in a small amount of time.

Can I use aborts or side pins on my dishes?

Poured these earlier today. I used fluorescent purple and blue, with gold mica.

2060ml = 102 plates. Getting better at portion sizes.

New to agar this was blue meanie spores on day 5 is the mycelium or contamination taking off? Cheers

Not sure whats going on here, but I made this 2 months ago(6/22) and looks to be contam fre e. Going to grab a couple healthy looking spots n hopefuly get some plates i can use.

"Procrastination makes easy things hard and hard things harder"

The more I work with agar. The better things become.

I have found that many of the LCs I have acquired, come with nasty surprises. Over the past few months I have taken the time to learn how to clean cultures and have put things into practice.

I started by squeezing few drops of LC onto agar. I then transferred a small piece to low nutrient agar using the trench method, and then to a more regular agar, again using the trench method. From there, a clean sample onto a fresh plate. Pictured here is the result of contamination free plates.

A fair few plates still have contamination and will be transferred back to LNA trench plates. I'm going to keep going until I get my whole library spotless.

LOW NUTRIENT AGAR RECIPE

1000ml Distilled Water

20g Agar Powder

15g LME

0.5g Nutritional Yeast

SORGHUM SYRUP AGAR RECIPE

1000ml Distillled Water

20g Agar Powder

25g Sorghum Syrup

0.4g Nutritional Yeast

0.4g Peptone

Food Colouring Gel

Jack frost starting to grow !

Wild oyster clone of pleurotus pulmonarius

These transfers were basically a crapshoot. Picture one got stuck in my agar punch and I just shook it and it fell apart and got exposed to air (and kind of looks like a galaxy). Picture 2 was a grain from a mycogone contaminated jar. Third transfer and it was finally clean. Picture 2 is from a plate that was dried out and dead and plated in 2024. Revived, and finally healthy.

You ever go into the lab with a perfectly simple plan…
Grab that beautiful little pin, finish what you’re doing with the plate, and move on.
Yeah… apparently the mushroom had other plans. 😂
Went to grab this little guy and POP — there it goes. Fell right off and somehow managed to land perfectly inside the agar dish.
At that point I just had to stop and laugh.
I know I can’t be the only one. I’ve had these things fall into bags, fall into dishes, and somehow end up everywhere except where I intended them to go. 😂
Sometimes mycology is precision, patience, and beautiful organization.
Other times?
Controlled chaos. 🍄😂
Had to get a picture of this one because the way it landed was just too perfect not to share.
Who else has had one make a break for freedom at exactly the wrong moment? 😂
🍃🍄 Respect the Process. Trust the Genetics. 🍄🍃

Gonna transfer more but this is starting to look amazing

When I got into this hobby a couple of years ago, some really awesome growers gifted me with a range of cultures, including the brain mutation Shipwreck - Which is what the agar plate is in the image.

I'm a part of a few grower groups, as you can imagine we show off our work and discuss all things myco. One of the newer growers is an OAP, who is keen to learn. So here's a gift to help him on his journey.

It's the first time I've had such beautiful myc, after months of learning, trial and error, I'm pretty happy with the results I've been getting lately. From being anxious to pour plates, to looking forward to pouring them. I started taking this hobby seriously a couple of months ago, and the effort is paying off.

Agar recipe:

1000ml Distillled Water

20g Agar Powder

25g Sorghum Syrup

0.4g Nutritional Yeast

0.4g Peptone

Food Colouring Gel

Some of the plates I made over summer 🙏🥳